Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity.
Cyclin-dependent kinase activity enhances phosphatidylcholine biosynthesis in Arabidopsis by repressing phosphatidic acid phosphohydrolase activity.
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DOI：
10.1111/tpj.13321
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Eastmond PJ
中科院分区：
文献类型：
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作者：
Craddock CP;Adams N;Kroon JT;Bryant FM;Hussey PJ;Kurup S;Eastmond PJ
内膜生物合成与细胞周期进程的协调被认为是在生长和发育过程中维持细胞功能的重要因素。我们以前表明，磷脂酸磷酸水解酶（PAH）的活动在拟南芥中的中断刺激主要磷脂磷脂酰胆碱（PC）的生物合成，并导致内质网扩张。在这里，我们表明，PC生物合成被核心细胞周期调节因子细胞周期蛋白依赖性激酶A;1（CDKA;1）的破坏所抑制，并且这种抑制依赖于PAH。此外，我们发现细胞周期蛋白依赖性激酶（CDK）在丝氨酸162处磷酸化PAH 1，这降低了其活性和膜结合。具有丝氨酸162至丙氨酸取代的PAH 1的CDK不敏感版本的表达抑制PC生物合成，并且还降低早期叶发育中的细胞分裂速率。总之，我们的研究结果揭示了一个生理上重要的机制，耦合率磷脂的生物合成和内膜生物合成细胞周期进程在拟南芥。随着细胞在整个细胞周期中的进展，协调内膜生物合成是重要的。在这里，我们发现了一个细胞周期蛋白依赖性激酶A;1调节机制，耦合磷脂生物合成和内膜生物合成的速度。
Coordination of endomembrane biogenesis with cell cycle progression is considered to be important in maintaining cell function during growth and development. We previously showed that the disruption of PHOSPHATIDIC ACID PHOSPHOHYDROLASE (PAH) activity in Arabidopsis thaliana stimulates biosynthesis of the major phospholipid phosphatidylcholine (PC) and causes expansion of the endoplasmic reticulum. Here we show that PC biosynthesis is repressed by disruption of the core cell cycle regulator CYCLIN‐DEPENDENT KINASE A;1 (CDKA;1) and that this repression is reliant on PAH. Furthermore, we show that cyclin‐dependent kinases (CDKs) phosphorylate PAH1 at serine 162, which reduces both its activity and membrane association. Expression of a CDK‐insensitive version of PAH1 with a serine 162 to alanine substitution represses PC biosynthesis and also reduces the rate of cell division in early leaf development. Together our findings reveal a physiologically important mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis to cell cycle progression in Arabidopsis. Coordinating endomembrane biogenesis as cells progress thorough the cell cycle is important. Here we uncover a CYCLIN‐DEPENDENT KINASE A;1 regulatory mechanism that couples the rate of phospholipid biosynthesis and endomembrane biogenesis.