DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.
DNA-binding activity of the adenovirus-induced E4F transcription factor is regulated by phosphorylation.
复制标题
腺病毒诱导的 E4F 转录因子的 DNA 结合活性受磷酸化调节。
DOI：
10.1101/gad.3.5.620
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发表时间：
1989
影响因子：
10.5
通讯作者：
Nevins,JR
中科院分区：
文献类型：
--
作者：
Raychaudhuri,P;Bagchi,S;Nevins,JR
先前的实验已经鉴定了E4F，一种可诱导的细胞因子，其结合腺病毒E4启动子中对E1A依赖性转录激活至关重要的序列。E4F因子已被纯化，并显示出在体外刺激E4启动子的转录。亲和纯化的因子的分析鉴定了具有E4F特异性结合活性的50 kD的单一多肽。E4F结合活性在F9细胞分化期间也受到调节，并且可以通过病毒感染在分化的F9细胞中被激活。此外，活化过程似乎涉及磷酸化事件，因为用碱性磷酸酶处理E4F会消除活性，而用病毒感染细胞的提取物孵育磷酸酶失活的因子会恢复活性。
Previous experiments have identified E4F, an inducible cellular factor that binds to sequences in the adenovirus E4 promoter that are critical for E1A-dependent transcriptional activation. The E4F factor has been purified and shown to stimulate transcription in vitro from the E4 promoter. Analysis of the affinity-purified factor identifies a single polypeptide of 50 kD that has E4F-specific binding activity. E4F binding activity is also regulated during F9 cell differentiation and can be activated in differentiated F9 cells by viral infection. Furthermore, the activation process appears to involve a phosphorylation event, because treatment of E4F with alkaline phosphatase abolishes activity and incubation of the phosphatase-inactivated factor with an extract from virus infected cells restores activity.