Evaluation of the protective effects of DNA vaccines encoding an infection-related cysteine protease from Cryptocaryon irritans, the pathogenic organism of cryptocaryoniasis
Evaluation of the protective effects of DNA vaccines encoding an infection-related cysteine protease from Cryptocaryon irritans, the pathogenic organism of cryptocaryoniasis
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编码来自隐核虫（隐核虫病的病原生物）感染相关半胱氨酸蛋白酶的 DNA 疫苗的保护作用评估
DOI：
10.1016/j.aquaculture.2021.737641
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发表时间：
2022
期刊：
影响因子：
4.5
通讯作者：
Tomoyoshi Yoshinaga
中科院分区：
文献类型：
--
作者：
Yuho Watanabe;Yuzo Takada;Maho Kotake;Kosuke Zenke;Naoki Itoh;Tomoyoshi Yoshinaga
由隐核虫刺激物引起的隐核虫病是热带和亚热带水域海水网箱养殖的主要威胁；然而，控制这种疾病仍然具有挑战性。在这项研究中，我们构建了编码 C 的半胱氨酸蛋白酶的 DNA 疫苗。刺激物（pcDNA3.1-cp2-full-myc 和 pcDNA3.1-cp2-partial-myc）并检查了疫苗的保护功效。攻毒实验结果表明，DNA疫苗免疫鱼体内的寄生虫数量低于对照鱼（注射磷酸盐缓冲盐水组和模拟载体注射组）；当使用 pcDNA3.1-cp2-full-myc 进行疫苗接种时，这种差异具有统计学意义（p < 0.05）。此外，发现半胱氨酸蛋白酶在不同的寄生虫分离株中相对保守。因此，该蛋白酶可能是开发针对多种艰难梭菌菌株的疫苗的潜在候选抗原。刺激者。
Cryptocaryoniasis, caused byCryptocaryon irritans, is a major threat to marine cage culture in tropical and subtropical waters; however, controlling the disease remains challenging. In this study, we constructed DNA vaccines encoding a cysteine protease ofC. irritans(pcDNA3.1-cp2-full-myc and pcDNA3.1-cp2-partial-myc) and examined the protective efficacy of the vaccines. The results of the challenge experiment showed that the number of parasites recovered from fish immunized with the DNA vaccines was lower than that recovered from control fish (phosphate-buffered saline-injected and mock vector-injected groups); this difference was statistically significant when pcDNA3.1-cp2-full-myc was used for vaccination (p< 0.05). In addition, the cysteine protease was found to be relatively conserved among different isolates of the parasite. Thus, the protease may be a potential antigen candidate for the development of a vaccine against multiple strains ofC. irritans.