A NEW METHOD FOR PREPARING FLAVIN-ADENINE DINUCLEOTIDE
A NEW METHOD FOR PREPARING FLAVIN-ADENINE DINUCLEOTIDE
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DOI：
10.1042/bj0540437
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发表时间：
1953-01-01
影响因子：
4.1
通讯作者：
WHITBY, LG
中科院分区：
文献类型：
--
作者：
WHITBY, LG
在粉末纤维素上的层析用于制备纯度为90%的黄素腺嘌呤二核苷酸（FAD），其不受其它黄素的污染和不受吸收240和510 m[mu]之间的光的化合物的污染。重新研究了FAD的分子消光系数。的可能性进行了讨论，在pH值为7.0的FAD的吸收减少，相对于其组分（黄素单甘肽和腺苷-5 [图像]-磷酸）的组合吸收，类似于淬灭的荧光FAD在类似的条件下，是由于形成一个内部复合物。
Chromatography on powdered cellulose was used to prepare flavinadenine dinucleotide (FAD) of 90% purity, free from contamination by other flavins and from contamination by compounds which absorb light between 240 and 510 m[mu]. The molecular extinction coefficients of FAD were reinvestigated. The possibility is discussed that the diminution in the absorption of FAD, at pH 7.0, relative to the combined absorption of its components (flavin mononucleotide and adenosine-5[image]-phosphate), is analogous to the quenching of fluorescence of FAD under similar conditions and is due to the formation of an internal complex.