Lavender Essential Oil and Its Main Constituents Inhibit the Expression of TNF-α-induced Cell Adhesion Molecules in Endothelial Cells
Lavender Essential Oil and Its Main Constituents Inhibit the Expression of TNF-α-induced Cell Adhesion Molecules in Endothelial Cells
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DOI：
10.18926/amo/55586
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发表时间：
2017-12-01
影响因子：
0.5
通讯作者：
Kataoka, Mikio
中科院分区：
文献类型：
--
作者：
Aoe, Michinori;Ueno-Iio, Tomoe;Kataoka, Mikio
熏衣草精油（Lvn）在卵清蛋白致敏的哮喘小鼠模型中具有抗炎作用，并抑制炎性细胞浸润到肺部。Lvn对细胞粘附分子的抗炎作用尚不清楚。在这里，我们评估了Lvn及其主要成分乙酸芳樟酯（LA）和芳樟醇（LO）对肿瘤坏死因子-α（TNF-α）诱导的细胞粘附分子在小鼠脑内皮bEnd.3细胞和人脐静脉内皮细胞（HUVECs）中表达的影响。用Lvn、LA或LO处理bEnd.3细胞，随后用TNF-α刺激。RT-PCR检测细胞粘附分子mRNA的表达水平。Western blotting检测E-选择素、P-选择素蛋白和NF-κ B p65磷酸化。RT-PCR检测Lvn对HUVECs的影响。在bEnd.3细胞中，Lvn和LA抑制TNF-α诱导的E-选择素、P-选择素、血管细胞粘附分子-1、细胞间粘附分子-1和细胞核中的磷酸化NF-κ B p65; LO不抑制P-选择素或磷酸化NF-κ B p65。Lvn抑制TNF-α诱导的HUVECs中E-选择素mRNA。这些结果表明，Lvn和LA通过抑制NF-κ B活化来抑制内皮细胞中TNF-α诱导的细胞粘附分子。因此，Lvn或包括LA在内的其他精油可用作替代抗炎药物。
Lavender essential oil (Lvn) has anti-inflammatory effects in an ovalbumin-sensitized murine model of asthma, and inhibits inflammatory cell infiltration into the lungs. The anti-inflammatory effects of Lvn on cell adhesion molecules are not clear. Here we evaluated the effects of Lvn and its main constituents, linalyl acetate (LA) and linalool (LO), on the expression of tumor necrosis factor-alpha (TNF-alpha)-induced cell adhesion molecules in murine brain endothelial bEnd.3 cells and human umbilical vein endothelial cells (HUVECs). The bEnd.3 cells were treated with Lvn, LA, or LO and subsequently stimulated with TNF-alpha. The mRNA expression levels of cell adhesion molecules were detected using RT-PCR. E-selectin and P-selectin protein and phosphorylated-NF-kappa B p65 were detected by western blotting. The effects of Lvn on HUVECs were measured by RT-PCR. In bEnd.3 cells, Lvn and LA suppressed TNF-alpha-induced E-selectin, P-selectin, vascular cell adhesion molecule-1, intercellular adhesion molecule-1, and phosphorylated-NF-kappa B p65 in the nucleus; LO did not suppress P-selectin or phosphorylated-NF-kappa B p65. Lvn inhibited TNF-alpha-induced E-selectin mRNA in HUVECs. These results indicate that Lvn and LA inhibit TNF-alpha-induced cell adhesion molecules in endothelial cells through the suppression of NF-kappa B activation. Consequently, Lvn or other essential oils including LA may be useful as alternative anti-inflammatory medicines.