POLYMORPHISM OF APOLIPOPROTEIN-A-II (APOA-II) AMONG INBRED STRAINS OF MICE - RELATIONSHIP BETWEEN THE MOLECULAR TYPE OF APOA-II AND MOUSE SENILE AMYLOIDOSIS
POLYMORPHISM OF APOLIPOPROTEIN-A-II (APOA-II) AMONG INBRED STRAINS OF MICE - RELATIONSHIP BETWEEN THE MOLECULAR TYPE OF APOA-II AND MOUSE SENILE AMYLOIDOSIS
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DOI：
10.1042/bj2790427
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发表时间：
1991-10-15
影响因子：
4.1
通讯作者：
TAKEDA, T
中科院分区：
文献类型：
--
作者：
HIGUCHI, K;KITAGAWA, K;TAKEDA, T
根据载脂蛋白A-II（apoA-II）cDNA的核苷酸序列预测了三种类型的apoA-II蛋白（A、B和C）。在4个位置观察到氨基酸残基的取代（A型：Pro-5、Asp-20、Met-26、Ala-38; B：Pro-5、Glu-20、瓦尔-26、瓦尔-38; C：Gln-5、Glu-20、瓦尔-26、Ala-38）。通过PCR消化扩增的apoA-II DNA，使用apoA-II基因的限制性内切酶（Cfr 13 I和Msp I）的限制性片段长度多态性，可鉴定每种类型。apoA-II的分子类型在23种小鼠中确定，包括我们实验室开发的9种衰老加速小鼠系列。在F2和F3杂交小鼠中apoA-II和淀粉样蛋白沉积类型的检查表明，apoA-II淀粉样蛋白沉积仅存在于12-17月龄的C型apoA-II纯合子小鼠中。apoA-II的分子类型可能是参与小鼠老年性淀粉样变的一个因素。
Three types of apolipoprotein A-II (apoA-II) proteins (A, B and C) were predicted from the nucleotide sequence of apoA-II cDNA. Substitution of amino acid residues was noted at four positions (type A: Pro-5, Asp-20, Met-26, Ala-38; B: Pro-5, Glu-20, Val-26, Val-38; C: Gln-5, Glu-20, Val-26, Ala-38). Each type was identifiable by digestion of amplified apoA-II DNA by PCR, using restriction-fragment-length polymorphism of the apoA-II gene for restriction enzymes (Cfr13I and MspI. The molecular type of apoA-II was determined among 23 strains of mice including nine of the senescence accelerated mouse series developed in our laboratory. Examination of types of apoA-II and amyloid deposition in the F2 and F3 hybrid mice showed that apoA-II amyloid deposition was present only in the mice homozygous for type C apoA-II and which were 12-17 months of age. The molecular type of apoA-II may be a factor involved in the development of senile amyloidosis in mice.