The Swi2/Snf2 Bromodomain Is Important for the Full Binding and Remodeling Activity of the SWI/SNF Complex on H3-and H4-acetylated Nucleosomes
The Swi2/Snf2 Bromodomain Is Important for the Full Binding and Remodeling Activity of the SWI/SNF Complex on H3-and H4-acetylated Nucleosomes
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DOI：
10.1196/annals.1414.038
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Hassan, Ahmed H.
中科院分区：
文献类型：
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作者：
Awad, Salma;Hassan, Ahmed H.
SWI/SNF染色质重塑复合体在其Swi2/Snf2亚基中包含一个溴域，有助于将其拴在乙酰化的启动子核小体上。为了研究这个溴结构域在SWI/SNF复合体中的重要性，我们将SWI/SNF的核小体结合和染色质重塑活性与缺乏Swi2/Snf2溴结构域的突变复合体进行了比较。在这里，我们证明了Swi2/Snf2溴结构域缺失的SWI/SNF复合体不能与SAGA-或NuA4-乙酰化的核小体以及野生型复合体结合。此外，我们还表明，这种减少的结合导致了这些乙酰化核小体模板的部分重构的Delta溴域SWI/SNF复合体。这些结果表明，Swi2/Snf2溴域是SWI/SNF复合体与H3-和H4-乙酰化核小体完全结合和功能活性所必需的。
The SWI/SNF chromatin-remodeling complex contains a bromodomain in its Swi2/Snf2 subunit that helps tether it to acetylated promoter nucleosomes. To study the importance of this bromodomain in the SWI/SNF complex, we have compared the nucleosome-binding and the chromatin-remodeling activities of the SWI/SNF to a mutant complex that lacks the Swi2/Snf2 bromodomain. Here we show that the SWI/SNF complex deleted of the Swi2/Snf2 bromodomain cannot bind to SAGA- or NuA4-acetylated nucleosomes as well as the wild-type complex. Moreover, we show that this reduced binding leads to partial remodeling of these acetylated nucleosome templates by the Delta bromodomain SWI/SNF complex. These results demonstrate that the Swi2/Snf2 bromodomain is required for the full binding and functional activity of the SWI/SNF complex on H3- and H4-acetylated nucleosomes.