MORPHOLOGICAL AND BIOCHEMICAL-CHARACTERIZATION OF INFLUENZA VACCINES COMMERCIALLY AVAILABLE IN THE UNITED-KINGDOM
MORPHOLOGICAL AND BIOCHEMICAL-CHARACTERIZATION OF INFLUENZA VACCINES COMMERCIALLY AVAILABLE IN THE UNITED-KINGDOM
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DOI：
10.1016/0264-410x(94)90227-5
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发表时间：
1994-06-01
期刊：
影响因子：
5.5
通讯作者：
WATTS, A
中科院分区：
文献类型：
--
作者：
RENFREY, S;WATTS, A
在英国有四种疫苗可用于预防流感病毒。所有这些都是亚单位疫苗，定义为裂解病毒体或纯化的表面抗原疫苗;每种不同类型有两种可用。两种疫苗类型的反应原性均低于全灭活病毒，其抗原性由病毒表面糖蛋白诱导。在这里，四种疫苗中的每一种都通过电子显微镜和SDS-PAGE分析进行了表征，得到了独特的疫苗谱。三种疫苗含有内部病毒核蛋白，在存在残留血凝素的情况下，可能诱导甲型流感病毒交叉反应性细胞毒性T细胞应答，因此对疫苗有效性具有价值。残留脂质存在于三种疫苗中，最近的证据表明，致热原性与存在具有表面糖蛋白簇的病毒脂质相关。通过结合电子显微镜证据和生化表征，不仅可以解决疫苗类型之间的成分差异，而且可以解决目前可用的每种疫苗之间的成分差异。因此，此处描述的形态学差异可能有助于疫苗接种后的潜在反应原性效应。
Four vaccines are available in the United Kingdom against influenza virus. All are subunit vaccines, defined as either split-virion or purified surface antigen vaccine; there are two of each distinct type available. Both vaccine types are less reactogenic than whole inactivated virus, with antigenicity induced by viral surface glycoproteins. Here, each of the four vaccines has been characterized by electron microscopy and SDS-PAGE analysis, giving a unique vaccine profile. Three vaccines contain internal viral nucleoprotein which, in the presence of residual haemagglutinin, may induce an influenza A virus cross-reactive cytotoxic T-cell response and thus be of value to vaccine effi;cacy. Residual lipid was present in three vaccines and recent evidence suggests that pyrogenicity is correlated with the presence of viral lipid with clusters of surface glycoproteins. By a combination of electron microscopic evidence and biochemical characterization, it has been possible to resolve compositional differences, not only between vaccine type, but also between each individual currently available vaccine. Hence, there is the possibility that the morphological differences characterized here may be contributory to potential reactogenic effects subsequent to vaccination.