GROWTH OF A RAT NEUROBLASTOMA CELL LINE IN SERUM-FREE SUPPLEMENTED MEDIUM
GROWTH OF A RAT NEUROBLASTOMA CELL LINE IN SERUM-FREE SUPPLEMENTED MEDIUM
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DOI：
10.1073/pnas.76.1.514
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
SATO, GH
中科院分区：
文献类型：
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作者：
BOTTENSTEIN, JE;SATO, GH
大鼠神经母细胞瘤B104细胞系起源于中枢神经系统，在添加胰岛素、转铁蛋白、黄体酮、硒和腐胺的合成培养液中，在无血清的情况下仍能增殖。当单独添加时，每种补充剂几乎没有影响；联合使用时，对细胞数量有显著的协同效应。细胞在该培养液中达到与添加10%胎牛血清的培养液中相同的饱和密度。在添加的培养基中观察到了更广泛的突起形成，并保留了其他分化特性。
The rat neuroblastoma B104 cell line, which originated in the CNS, was able to proliferate in the absence of serum in synthetic medium supplemented with insulin, transferrin, progesterone, Se and putrescine. When added individually, each supplement had little or no effect; in combination there was a marked synergistic effect on cell number. The cells attained the same saturation density in this medium as in medium with 10% fetal calf serum added. More extensive process formation was observed in the supplemented medium and other differentiated properties were retained as well.