Sequence of the Kluyveromyces lactis URA3 gene.
Sequence of the Kluyveromyces lactis URA3 gene.
复制标题
乳酸克鲁维酵母 URA3 基因的序列。
作者：
J. Shuster;D. Moyer;B. Irvine
我们在此报告了乳酸克鲁维菌 URA3 基因（乳清酸-5-磷酸脱羧酶）的序列。通过与 E. cdi 菌株 MNU（Michael Hall 赠送）中的pyrF::Tn5 突变互补，从 Sau3A K. lacuis 基因组文库中分离出该基因。分离的序列能够补充 K. lac*s 的 ura3 突变以及酿酒酵母的 ura3-52 突变。乳酸克鲁维酵母蛋白与其酿酒酵母类似物有 81% 同源性 (1)，DNA 序列有 71% 同源性。这些同源性水平和其他乳酸克鲁维酵母克隆基因（未发表的数据）的同源性标志着乳酸克鲁维酵母是迄今为止与酵母属最相关的酵母。 K.lactis 蛋白与类似的 Neurosjra cassa PYR4 蛋白有约 30% 同源性，DNA 序列有约 24% 同源性 (2)。
We report here the sequence of the Kluyvercuyces lactis URA3 gene (orotidine-5-phoshate decarboxylase). The gene was isolated from a Sau3A K. lacuis genomic library by complementation of a pyrF::Tn5 mutation in E. cdi strain MNU (gift from Michael Hall). The isolated sequence was able to complement a ura3 mutation of K. lac*s as well as the ura3-52 mutation in S. cerevisiae. The K. lactis protein is 81 percent homologous to its S. cerevislae analog (1) and the DNA sequences are 71 percent homologous. These levels of homology and those of other K. lactis cloned genes (unpublished data) mark K. lacts as the yeast most related to the Sacdharomyces genus to date. The K. lactis protein is about 30 percent homologous to the analogous Neurosjra cassa PYR4 protein and the DNA sequences are about 24 percent homologus (2).