Protective effects of repetitive transcranial magnetic stimulation in a rat model of transient cerebral ischaemia: a microPET study
Protective effects of repetitive transcranial magnetic stimulation in a rat model of transient cerebral ischaemia: a microPET study
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DOI：
10.1007/s00259-009-1342-3
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发表时间：
2010-05-01
影响因子：
9.1
通讯作者：
Zhang, Hong
中科院分区：
文献类型：
--
作者：
Gao, Feng;Wang, Shuang;Zhang, Hong
重复经颅磁刺激（rTMS）是一种非侵入性的方法来兴奋大脑中的神经元。然而，其治疗中风的潜在机制仍不清楚。本研究的目的是使用正电子发射断层扫描（PET）研究高频rTMS在短暂性脑缺血大鼠模型中的神经保护作用。Sprague-Dawley大鼠（n=30）用水合氯醛麻醉并进行90分钟的管腔内大脑中动脉闭塞（MCAO），随后分为三组：对照组（n=10）、rTMS组（n=10）或假rTMS组（n=10）。rTMS组于缺血后1h及MCAO后24 h每隔1次给予rTMS，共7天。在所有三组中，使用F-18-FDG的小动物PET（microPET）成像来评估脑葡萄糖代谢。免疫组化染色检测梗死边缘细胞凋亡分子，rTMS组大鼠神经功能评分在整个7 d观察期内均高于对照组。总的，皮质和纹状体梗死体积显着小于rTMS组比对照组，如2，3，5-氯化三苯基四氮唑染色。F-18-FDG microPET图像显示，在受影响的半球，rTMS组的皮质和纹状体的标准化摄取值显著高于对照组。rTMS组caspase-3阳性细胞数明显低于对照组，而Bcl-2/Bax比值明显高于对照组，rTMS治疗增加了缺血半球的糖代谢，抑制了细胞凋亡。在动物研究和未来的临床试验中，F-18-FDG PET可用于监测短暂性脑缺血的rTMS治疗。
Repetitive transcranial magnetic stimulation (rTMS) is a noninvasive method to excite neurons in the brain. However, the underlying mechanism of its therapeutic effects in stroke remains unclear. The aim of this study was to investigate the neuroprotective effect of high-frequency rTMS in a rat model of transient cerebral ischaemia using positron emission tomography (PET).Sprague-Dawley rats (n=30) were anaesthetized with chloral hydrate and subjected to 90 min of intraluminal middle cerebral artery occlusion (MCAO) with subsequent reperfusion in three groups: control (n=10), rTMS (n=10), or sham-rTMS groups (n=10). In the rTMS group, rTMS was given 1 h after ischaemia and every 24 h for 7 days after MCAO. In all three groups, small-animal PET (microPET) imaging with F-18-FDG was used to evaluate brain glucose metabolism. Apoptotic molecules were measured in the infarct margin using immunohistochemical staining.The neurological scores of the rats in the rTMS group were higher than in those of the control group over the whole 7-day observation period. The total, cortical and striatal infarct volumes were significantly less in the rTMS group than in the control group, as measured by 2,3,5-triphenyltetrazolium chloride staining. F-18-FDG microPET images showed significantly higher standardized uptake values in the cortex and striatum in the rTMS group than in the control group in the affected hemisphere. The number of cells positive for caspase-3 was significantly lower in the rTMS group than in the control group, while the Bcl-2/Bax ratio was significantly higher in the rTMS group than in the control group.rTMS therapy increased glucose metabolism and inhibited apoptosis in the ischaemic hemisphere. F-18-FDG PET could be used to monitor rTMS therapy in transient cerebral ischaemia in animal studies and in future clinical trials.