Genetic control of steroidogenesis and spermatogenesis in inbred mice.

Genetic control of steroidogenesis and spermatogenesis in inbred mice.
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近交小鼠类固醇生成和精子生成的遗传控制。

DOI:
10.1111/j.1749-6632.1984.tb38322.x
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发表时间:
1984
影响因子:
5.2
通讯作者:
Nolan,C
Nolan,C
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Chubb,C;Nolan,C

文献摘要

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MATERIALS AND METHODSInbred male mice (F33) were purchased from the Jackson Laboratory, Bar Harbor, ME. The mice were siblings and were either mutants homozygous for the at gene mutation (atlat) or normal controls (? I+). Sibling mice were housed together and supplied with feed and water ad libitum. The animal room was maintained at 23+ 2" C with 14 hr light/24 hr.Mice (9-14 weeks old) were sacrificed by cervical dislocation, selected organs weighed, and the testes trimmed of adnexa. Intact testes were either perfused in vitro* or perfusion-fixed with 2% glutaraldehyde in 0.1 M cacodylate buffer and embedded in metha~ rylate.~ Randomly selected sections of testes were stained with toluidine blue.