Use of 13C-labeled glucose for measuring exogenous glucose oxidation in mice
Use of 13C-labeled glucose for measuring exogenous glucose oxidation in mice
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DOI：
10.1271/bbb.66.426
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发表时间：
2002-02-01
影响因子：
1.6
通讯作者：
Fushiki, T
中科院分区：
文献类型：
--
作者：
Ishihara, K;Oyaizu, S;Fushiki, T
作者改装了呼吸气体分析仪，对12只小型实验动物的呼吸（CO2）-C-13进行了一次分析。为了研究该系统的实际应用，小鼠口服（OR）或静脉内（IV）给予含有三种不同量的C-13标记葡萄糖的葡萄糖溶液。在OR小鼠中，在给药后10分钟内检测到外源性葡萄糖衍生的过期（CO2）-C-13，但在IV小鼠中约30分钟。（CO2）-C-13呼气峰的高度与给予的C-13-葡萄糖质量相关。
The author modified a respiratory gas analyzer to analyze the respiratory (CO2)-C-13 Of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of C-13-labeled glucose. Expired (CO2)-C-13 derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in IV mice. The height of the peak of (CO2)-C-13 expiration was correlated with the administered C-13-glucose mass.