COMPARISON OF EFFECTOR CELLS INVOLVED IN CELL-MEDIATED LYMPHOLYSIS AND ANTIBODY - DEPENDENT CELL-MEDIATED CYTOTOXICITY IN MAN
COMPARISON OF EFFECTOR CELLS INVOLVED IN CELL-MEDIATED LYMPHOLYSIS AND ANTIBODY - DEPENDENT CELL-MEDIATED CYTOTOXICITY IN MAN
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DOI：
10.1111/j.1365-3083.1975.tb02609.x
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发表时间：
1975-01-01
影响因子：
3.7
通讯作者：
BONNARD, GD
中科院分区：
文献类型：
--
作者：
MACDONALD, HR;BONNARD, GD
比较了细胞毒性淋巴细胞和抗体依赖性杀伤细胞的一些物理和机械特性。采用细胞介导淋巴溶解（CML）技术对单向混合白细胞培养（MLC）中人外周血白细胞（PBL）致敏后的囊毒性淋巴细胞进行检测。在抗体依赖细胞介导的细胞毒性（ADCMC）实验中，以51cr标记的小鼠肥大细胞瘤细胞为靶标，在MLC前后测量杀伤细胞。对1 g流速沉降分离的细胞毒活性进行定量分析表明，MLC中的细胞毒淋巴细胞是一个相对异质性的快速沉积细胞群体，在形态上与淋巴母细胞相对应。在MLC之前分离PBL的研究表明，这种细胞毒性淋巴细胞来源于小淋巴细胞祖细胞。分离群体的ADCMC分析表明，PBL中大多数杀伤细胞具有小淋巴细胞的沉降特性。MLC中PBL刺激后，ADCMC活性升高；活性的增加与快速沉积细胞的数量有关。比较细胞毒性淋巴细胞和杀伤细胞对EDTA、细胞松弛素B和二甲亚砜抑制的敏感性，两种效应细胞间差异无统计学意义。
Some physical and mechanistic properties of cytotoxic lymphocytes and antibody-dependent killer cells were compared. Cystotoxic lymphocytes were measured by the cell-mediated lympholysis (CML) technique after sensitization of human peripheral blood leukocytes (PBL) in unidirectional mixed leukocyte culture (MLC). Killer cells were measured before and after MLC in an antibody-dependent cell-mediated cytotoxicity (ADCMC) assay using 51Cr-labeled mouse mastocytoma cells as targets. Quantitative analysis of the cytotoxic activity of populations separated by velocity sedimentation at 1 g indicated that cytotoxic lymphocytes in MLC were a relatively heterogeneous population of rapidly sedimenting cells that corresponded morphologically to lymphoblasts. Studies in which PBL were separated before MLC suggested that such cytotoxic lymphocytes were derived from small lymphocyte progenitors. Analysis of ADCMC in separated populations showed that most killer cells in PBL had the sedimentation properties of small lymphocytes. After stimulation of PBL in MLC, increased ADCMC activity was detected; the increased activity was associated with population of rapidly sedimenting cells. Comparison of the sensitivity of cytotoxic lymphocytes and killer cells to inhibition by EDTA, cytochalasin B and dimethyl sulfoxide showed no significant differences between the 2 types of effector cells.