Effects of fixatives on histomagnetic evaluation of iron in rodent spleen.
Effects of fixatives on histomagnetic evaluation of iron in rodent spleen.
复制标题
固定剂对啮齿动物脾脏中铁的组织磁学评价的影响。
DOI：
10.1016/j.jmmm.2020.167531
复制
发表时间：
2021
影响因子：
2.7
通讯作者：
Agarwal,Gunjan
中科院分区：
文献类型：
--
作者：
Walsh,KevinJ;Shah,StavanV;Wei,Ping;Oberdick,SamuelD;Karn,NicoleM;McTigue,DanaM;Agarwal,Gunjan
确定铁在组织切片中的分布对于几种病理来说很重要。切除组织中的铁含量通常通过组织化学染色进行分析，这取决于样品准备和染色方案。在我们最近的研究中，我们研究了如何利用铁的磁性来以无标记的方式表征组织切片中的铁分布。为了能够对各种可用组织中的铁进行组织磁学表征，重要的是将其扩展到常规准备用于组织化学染色的样品，这通常涉及到使用化学固定剂。在这项研究中，我们采用了一种系统的方法来确定未固定和福尔马林固定的小鼠脾组织在铁的组织磁学特征方面的差异。利用超导量子干涉装置(SQUID)和磁力显微镜(MFM)进行了宏观和微观尺度的组织磁学表征。用Perl‘s染色对相邻切片上的铁(Fe3+)进行组织化学表征，用于MFM分析。虽然组织化学分析显示固定样本和未固定样本之间的染色分散度有显著差异，但组织磁学特征并不依赖于组织的化学固定。这项研究的结果表明，铁的组织磁性表征没有组织化学分析中可能出现的染色伪影。
Characterizing the iron distribution in tissue sections is important for several pathologies. Iron content in excised tissue is typically analyzed via histochemical stains, which are dependent on sample preparation and staining protocols. In our recent studies, we examined how magnetic properties of iron can also be exploited to characterize iron distribution in tissue sections in a label free manner. To enable a histomagnetic characterization of iron in a wide variety of available tissues, it is important to extend it to samples routinely prepared for histochemical staining, which often involve use of chemical fixatives. In this study, we took a systematic approach to determine differences between unfixed and formalin-fixed murine spleen tissues in histomagnetic characterization of iron. Superconducting quantum interference device (SQUID) magnetometry and magnetic force microscopy (MFM) were used for macro- and micro-scale histomagnetic characterization. Perl’s stain was used for histochemical characterization of ferric (Fe3+) iron on adjacent sections as that used for MFM analysis. While histochemical analysis revealed a substantial difference in the dispersion of the stain between fixed versus unfixed samples, histomagnetic characterization was not dependent on chemical fixation of tissue. The results from this study reveal that histomagnetic characterization of iron is free from staining artifacts which can be present in histochemical analysis.