TYPE-BETA TRANSFORMING GROWTH-FACTOR INHIBITS PROLIFERATION AND EXPRESSION OF ALKALINE-PHOSPHATASE IN MURINE OSTEOBLAST-LIKE CELLS
TYPE-BETA TRANSFORMING GROWTH-FACTOR INHIBITS PROLIFERATION AND EXPRESSION OF ALKALINE-PHOSPHATASE IN MURINE OSTEOBLAST-LIKE CELLS
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DOI：
10.1016/0006-291x(86)91057-0
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发表时间：
1986-10-15
影响因子：
3.1
通讯作者：
RODAN, GA
中科院分区：
文献类型：
--
作者：
NODA, M;RODAN, GA
TGF-.beta.调节许多细胞类型的生长和分化。MC 3 T3 E1是一种在培养物中分化的克隆非转化鼠骨细胞系。我们测试了猪TGF-β的作用。通过跟踪细胞数量和碱性磷酸酶活性，对单层培养的MC 3 T3 E1细胞的增殖和分化的影响。TGF-.beta.处理（2ng/ml）将MC 3 T3 E1细胞的形状从立方形改变为细长/纺锤形。TGF-.beta.以剂量依赖性方式抑制MC 3 T3 E1的生长高达40%（P < 0.02），其中在1 ng/ml时具有半数最大抑制。生长抑制依赖于血清浓度，最大抑制发生在2%血清。碱性磷酸酶的表达（其在体外当细胞达到汇合时达到峰值）被TGF-β强烈抑制，以剂量依赖性方式，在约0.05 ng/ml时具有半数最大抑制，在2 ng/ml时具有完全抑制。碱性磷酸酶抑制在暴露于TGF-β 24小时后是不可逆的。
TGF-.beta. modulates growth and differentiation in many cell types. MC3T3E1 is a clonal non-transformed murine bone cell line which differentiates in culture. We tested the effect of porcine TGF-.beta. on the proliferation and differentiation of MC3T3E1 cells in monolayer cultures by following cell number, and alkaline phosphatase activity. TGF-.beta. treatment (2 ng/ml) altered the shape of MC3T3E1 cells from cuboidal to elongated/spindle-shape. TGF-.beta. inhibited the growth of MC3T3E1 by up to 40% (P < 0.02) in a dose-dependent manner with half maximal inhibition at 1 ng/ml. Growth inhibition depended on serum concentration, maximal inhibition occurring at 2% serum. Expression of alkaline phosphatase, which peaks in vitro when the cells reach confluence, was strongly inhibited by TGF-.beta., in a dose-dependent manner with half maximal inhibition at around 0.05 ng/ml and complete inhibition at 2 ng/ml. Alkaline phosphatase inhibition was inrreversible after 24 hours exposure to TGF-.beta.