Quantitation of blood and plasma amino acids using isotope dilution electron impact gas chromatography/mass spectrometry with U-(13)C amino acids as internal standards.
Quantitation of blood and plasma amino acids using isotope dilution electron impact gas chromatography/mass spectrometry with U-(13)C amino acids as internal standards.
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DOI：
10.1002/(sici)1097-0231(19991115)13:21
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发表时间：
1999-11
期刊：
影响因子：
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通讯作者：
A. Calder;K. E. Gården;Susan E. Anderson;G. Lobley
中科院分区：
文献类型：
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作者：
A. Calder;K. E. Gården;Susan E. Anderson;G. Lobley
本文描述了一种同位素稀释气相色谱/质谱（GC/mS）定量测定血液和血浆氨基酸的方法。样品以U-(13)C氨基酸为内标，用毛细管柱气相色谱分离叔丁基二甲基硅基衍生物（tBDMS）。各氨基酸的线性回归曲线的相关系数为0.9999。通过对10个重复血液和血浆样本的分析，评估了该方法的可重复性。除天冬氨酸的变异系数为1.8%外，大多数氨基酸的变异系数(CV) </=1%。这可能是由于分析样品中这种氨基酸的浓度较低。添加到血浆中的氨基酸回收率在96 ~ 103%之间。电子冲击电离（EI）的使用使该方法可以在只有更基本的GC/mS可用的实验室中使用，并减少了仪器维护时间。该方法在需要精确和精确的氨基酸浓度的工作领域应该证明是有用的。
A method to quantitate blood and plasma amino acids by isotope dilution gas chromatography/mass spectrometry (GC/mS) is described. Samples were spiked with U-(13)C amino acids as internal standards and the tert-butyldimethylsilyl derivatives (tBDMS) separated by capillary column gas chromatography. Linear regression curves, generated for individual amino acids, gave correlation coefficients of 0.9999. The reproducibility of the method was assessed from the analysis of 10 replicate blood and plasma samples. For most amino acids a coefficient of variance (CV) of </=1% was obtained with the exception of aspartate which gave a value of 1.8%. This was probably due to the low concentration of this amino acid in the samples analysed. Recovery of amino acids added to plasma was between 96 and 103%. The use of electron impact ionization (EI) allows the method to be used in laboratories where only the more basic GC/mS is available and reduces the time spent on instrument maintenance. The method should prove useful in areas of work where accurate and precise amino acid concentrations are required.