QTL analysis of ergot resistance in sorghum
QTL analysis of ergot resistance in sorghum
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DOI：
10.1007/s00122-008-0781-8
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发表时间：
2008-05
影响因子：
5.4
通讯作者：
D. Parh;David Jordan;Elizabeth A. B. Aitken;E. Mace;P. Jun-ai;C. McIntyre;Ian D. Godwin
中科院分区：
文献类型：
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作者：
D. Parh;David Jordan;Elizabeth A. B. Aitken;E. Mace;P. Jun-ai;C. McIntyre;Ian D. Godwin
高粱麦角菌主要由非洲角菌（claviceps africanaFrederickson, Mantle, de Milliano）引起，是全球高粱产业的重大威胁。本研究的目的是首先确定与麦角菌抗性相关的分子标记以及花粉数量（PQ）和花粉活力（PV）两个花粉性状；其次，评估这两个花粉性状与高粱麦角菌抗性之间的关系。利用36个SSR、117个AFLP™、148个DArT™和2个形态性状位点的303个标记，构建了高粱RIL群体R931945-2-2 × IS 8525（抗性源）的遗传连锁图谱。复合区间定位鉴定出9个、5个和4个QTL分别与麦角百分比感染（PCERGOT）、PQ和PV分子标记相关，LOD为2.0。在4个染色体上发现了共定位/连锁QTL，而3个性状的其他QTL则是独立定位的，表明该高粱群体的麦角菌抗性机制在花粉和非花粉基础上都起作用。在为PCERGOT确定的9个QTL中，5个是使用整体数据集确定的，而4个是特定于由温度和湿度定义的组数据集。在SBI-02和SBI-06上鉴定的QTL在其他群体中得到进一步验证。这是高粱抗麦角菌相关QTL的首次报道。本文报道的标记可用于高粱这一重要病害的标记辅助选择。
Sorghum ergot, caused predominantly byClaviceps africanaFrederickson, Mantle, de Milliano, is a significant threat to the sorghum industry worldwide. The objectives of this study were firstly, to identify molecular markers linked to ergot resistance and to two pollen traits, pollen quantity (PQ) and pollen viability (PV), and secondly, to assess the relationship between the two pollen traits and ergot resistance in sorghum. A genetic linkage map of sorghum RIL population R931945-2-2 × IS 8525 (resistance source) was constructed using 303 markers including 36 SSR, 117 AFLP™, 148 DArT™ and two morphological trait loci. Composite interval mapping identified nine, five, and four QTL linked to molecular markers for percentage ergot infection (PCERGOT), PQ and PV, respectively, at a LOD >2.0. Co-location/linkage of QTL were identified on four chromosomes while other QTL for the three traits mapped independently, indicating that both pollen and non pollen-based mechanisms of ergot resistance were operating in this sorghum population. Of the nine QTL identified for PCERGOT, five were identified using the overall data set while four were specific to the group data sets defined by temperature and humidity. QTL identified on SBI-02 and SBI-06 were further validated in additional populations. This is the first report of QTL associated with ergot resistance in sorghum. The markers reported herein could be used for marker-assisted selection for this important disease of sorghum.