SEROTONIN IN BODY-FLUIDS - CHARACTERIZATION OF HUMAN PLASMATIC AND CEREBROSPINAL-FLUID POOLS BY MEANS OF A NEW HPLC METHOD
SEROTONIN IN BODY-FLUIDS - CHARACTERIZATION OF HUMAN PLASMATIC AND CEREBROSPINAL-FLUID POOLS BY MEANS OF A NEW HPLC METHOD
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DOI：
10.1016/0024-3205(85)90406-0
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发表时间：
1985-01-01
期刊：
影响因子：
6.1
通讯作者：
GELPI, E
中科院分区：
文献类型：
--
作者：
ARTIGAS, F;SARRIAS, MJ;GELPI, E
本文介绍了一种测定血浆和脑脊液中PM量5-羟色胺(5-羟色胺)的新高效液相色谱技术。以蟾酥宁为内标。检测是通过电化学或荧光法实现的。检测下限可估计为50 pg 5羟色胺/毫升(0.3 PM)。该方法被用来表征与血小板池明显不同的非颗粒性5-羟色胺。给大鼠注射对氯苯丙氨酸(PCPA)300 mg/kg可使血浆池减少90%，而血小板5-羟色胺仅轻微降低(PCPA后第3天)，甚至升高(PCPA后第7天)。人体血浆5-羟色胺浓度(n=15)为2.6±-。0.9 ng/ml(.×.+-。SD)。将该方法应用于神经科患者的脑脊液，结果显示5-羟色胺的浓度范围为93-962pg/ml。
A new HPLC [high-performance liquid chromatography] technique for the analysis of pM amounts of serotonin (5HT) in plasma and CSF is described. Bufotenin is used as internal standard. Detection is achieved electrochemically or fluorimetrically. The detection limit can be estimated as 50 pg 5HT/ml of either fluid (0.3 pM). The method is used to characterize a non-particulate pool of 5HT which is clearly distinct of the platelet pool. Administration of parachlorophenylalanine (PCPA) 300 mg/kg to rats leads to a 90% reduction in the plasmatic pool; platelet 5HT is only slightly decreased (3rd day after PCPA) or even increased (7th day after PCPA). Human concentration (n = 15) of 5HT in plasma is 2.6 .+-. 0.9 ng/ml (.times. .+-. SD). The application of the method to CSF of neurological patients reveals 5HT concentrations ranging from 93-962 pg/ml.