A Kinesin-14 Motor Activates Neocentromeres to Promote Meiotic Drive in Maize
A Kinesin-14 Motor Activates Neocentromeres to Promote Meiotic Drive in Maize
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DOI：
10.1016/j.cell.2018.03.009
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发表时间：
2018-05-03
期刊：
影响因子：
64.5
通讯作者：
Hiatt, Evelyn N.
中科院分区：
文献类型：
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作者：
Dawe, R. Kelly;Lowry, Elizabeth G.;Hiatt, Evelyn N.
玉米异常10号染色体(AB10)编码了一个典型的真正减数分裂驱动的例子，它将称为节点的异色区域转化为可移动的新着丝粒，并优先传递给卵细胞。在这里，我们确定了AB10上的八个基因，称为动蛋白驱动(KINDR)复合体，这是新着丝粒运动和优先传递所必需的。两个缺乏新着丝粒活性的减数分裂驱动突变体被证明是在整个基因簇上DNA甲基化增加的同源表变异体。KindR的RNAi导致了第三个表位突变和相应的减数分裂驱动的丧失。Kinesin滑动分析和免疫定位表明，KINDR是一种功能缺失的Kinesin，它特异地定位于含有180bp重复序列的旋钮。序列比较表明，Kindr偏离了类似于12 myA的kinesin-14A祖先，并推动了>；500Mb的节状重复序列的积累，并影响了全部10条染色体上数千个与节状结构相连的基因的分离。
Maize abnormal chromosome 10 (Ab10) encodes a classic example of true meiotic drive that converts heterochromatic regions called knobs into motile neocentromeres that are preferentially transmitted to egg cells. Here, we identify a duster of eight genes on Ab10, called the Kinesin driver (Kindr) complex, that are required for both neocentromere motility and preferential transmission. Two meiotic drive mutants that lack neocentromere activity proved to be kindr epimutants with increased DNA methylation across the entire gene cluster. RNAi of Kindr induced a third epimutant and corresponding loss of meiotic drive. Kinesin gliding assays and immunolocalization revealed that KINDR is a functional minus-enddir ted kinesin that localizes specifically to knobs containing 180 bp repeats. Sequence comparisons suggest that Kindr diverged from a Kinesin-14A ancestor similar to 12 mya and has driven the accumulation of > 500 Mb of knob repeats and affected the segregation of thousands of genes linked to knobs on all 10 chromosomes.