Genomic Features of High-Priority Salmonella enterica Serovars Circulating in the Food Production Chain, Brazil, 2000-2016
Genomic Features of High-Priority Salmonella enterica Serovars Circulating in the Food Production Chain, Brazil, 2000-2016
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DOI：
10.1038/s41598-019-45838-0
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发表时间：
2019-07-30
影响因子：
4.6
通讯作者：
Landgraf, Mariza
中科院分区：
文献类型：
--
作者：
Monte, Daniel F. .;Lincopan, Nilton;Landgraf, Mariza
多药耐药(MDR)肠炎沙门氏菌已被世界卫生组织列为高度优先的病原体。对从巴西家禽和猪生产链中回收的264株肠炎沙门氏菌进行了全基因组测序研究。属于28个血清型的大多数国际谱系，包括S.Schwarzengrund ST96、S.Typhimurium ST19、S.Minneota ST548、S.Infantis ST32、S.Heidelberg ST15、S.Newport ST45、S.Brandenburg ST65和S.Kentucky ST198，都具有MDR和毒力遗传背景。在这方面，耐药组分析显示存在qnrE1(首次在食物链鼠伤寒沙门氏菌中发现)、qnrB19、qnrS1、bla(CTX-M-8)、bla(CTX-M-2)和bla(CMY-2)基因以及gyrA突变；而ColpVC、IncHI2A、IncHI2、IncFIA、Incl1、Inca/C2、IncR、IncX1和po111质粒被检测到。此外，系统发育分析揭示了多个独立的谱系，如S.Enterica Serovars S.Infantis、S.Schwarzengrund、S.Minneota、S.Kentucky和S.Brandenburg。总之，肠链球菌国际谱系在食品生产链中的出现和持续是由保守的基因组和广泛的病毒组和抵抗组支持的。
Multidrug-resistant (MDR) Salmonella enterica has been deemed a high-priority pathogen by the World Health Organization. Two hundred and sixty-four Salmonella enterica isolates recovered over a 16-year period (2000 to 2016) from the poultry and swine production chains, in Brazil, were investigated by whole-genome sequencing (WGS). Most international lineages belonging to 28 serovars, including, S. enterica serovars S. Schwarzengrund ST96, S. Typhimurium ST19, S. Minnesota ST548, S. Infantis ST32, S. Heidelberg ST15, S. Newport ST45, S. Brandenburg ST65 and S. Kentucky ST198 displayed MDR and virulent genetic backgrounds. In this regard, resistome analysis revealed presence of qnrE1 (identified for the first time in S. Typhimurium from food chain), qnrB19, qnrS1, bla(CTX-M-8), bla(CTX-M-2) and bla(CMY-2) genes, as well as gyrA mutations; whereas ColpVC, IncHI2A, IncHI2, IncFIA, Incl1, IncA/C2, IncR, IncX1 and po111 plasmids were detected. In addition, phylogenetic analysis revealed multiple independent lineages such as S. enterica serovars S. Infantis, S. Schwarzengrund, S. Minnesota, S. Kentucky and S. Brandenburg. In brief, ocurrence and persistence of international lineages of S. enterica serovars in food production chain is supported by conserved genomes and wide virulome and resistome.