Activation of platelet-rich plasma using soluble type I collagen
Activation of platelet-rich plasma using soluble type I collagen
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DOI：
10.1016/j.joms.2007.06.635
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发表时间：
2008-04-01
影响因子：
1.9
通讯作者：
Murray, Martha M.
中科院分区：
文献类型：
--
作者：
Fufa, Duretti;Shealy, Blake;Murray, Martha M.
目的：富血小板血浆（PRP）最近被发现是一个有用的输送系统的生长因子重要的口腔组织愈合。但是，将液体形式的PRP应用于口腔内的伤口部位可能由于PRP大量损失到周围口腔空间中而变得复杂，除非通过凝血机制实现凝胶化。甘精胰岛素目前使用牛凝血酶完成;然而，这种方法罕见但严重的并发症导致了对替代凝血机制的研究，包括使用可溶性胶原蛋白作为凝血激活剂。在这项工作中，我们的假设是，可溶性I型胶原蛋白将作为有效的牛凝血酶在引起凝血的PRP和刺激生长因子从血小板和granulocyte.Materials和方法：PRP从人类捐助者凝块使用I型胶原蛋白或牛凝血酶。通过测量凝块直径随时间的变化来确定凝块回缩。释放血小板衍生生长因子（PDGF）-AB，转化生长因子（TGF）-β 1，和血管内皮生长因子（VEGF）从这两种类型的凝块测定超过10天，使用酶联免疫吸附assasy.Results：凝块形成使用I型胶原蛋白表现出远低于收缩比那些形成牛凝血酶。牛凝血酶和I型胶原蛋白刺激类似的释放PDGF-AB和VEGF之间的1和10天，然而，凝血酶激活导致更大的释放TGF-β 1在第一个5天后activation.Conclusions：使用I型胶原蛋白激活PRP凝血可能是一个安全和有效的替代牛凝血酶。与目前可用的凝块激活方法相比，胶原的使用导致较少的凝块收缩和相等的PDGF-AB和VEGF释放。(C)2008年美国口腔颌面外科医师协会。
Purpose: Platelet-rich plasma (PRP) has recently been found to be a useful delivery system for growth factors important to oral tissue healing. But application of PRP in a liquid form to a wound site within the oral cavity can be complicated by significant loss of the PRP into the surrounding oral space unless gelation through the clotting mechanism is accomplished. Gelation is currently accomplished using bovine thrombin; however, rare but serious complications of this method have led to the search for alternative clotting mechanisms, including the use of soluble collagen as a clotting activator. In this work, our hypothesis was that soluble type I collagen would be as effective as bovine thrombin in causing clotting of the PRP and stimulating growth factor release from the platelets and granulocytes.Materials and Methods: PRP from human donors was clotted using type I collagen or bovine thrombin. Clot retraction was determined by measuring clot diameters over time. The release of platelet-derived growth factor (PDGF)-AB, transforming growth factor (TGF)-beta 1, and vascular endothelial growth factor (VEGF) from both types of clots was measured over 10 days using enzyme-linked immunosorbent assasy.Results: Clots formed using type I collagen exhibited far less retraction than those formed with bovine thrombin. Bovine thrombin and type I collagen stimulated similar release of PDGF-AB and VEGF between 1 and 10 days; however, thrombin activation resulted in a greater release of TGF-beta 1 during the first 5 days after activation.Conclusions: The use of type I collagen to activate clotting of PRP may be a safe and effective alternative to bovine thrombin. The use of collagen results in less clot retraction and equal release of PDGF-AB and VEGF compared with currently available methods of clot activation. (C) 2008 American Association of Oral and Maxillofacial Surgeons.