Visualization of DNA replication on individual Epstein-Barr virus episomes
Visualization of DNA replication on individual Epstein-Barr virus episomes
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DOI：
10.1126/science.1064603
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发表时间：
2001-12-14
期刊：
影响因子：
56.9
通讯作者：
Schildkraut, CL
中科院分区：
文献类型：
--
作者：
Norio, P;Schildkraut, CL
哺乳动物基因组的复制是一个有组织的事件，但关于特定遗传位点复制程序的精确性的信息有限。我们开发了一种方法，可以在单个DNA分子中可视化DNA复制事件。研究EB病毒附加体的潜伏复制，我们发现，不同的起始位点被用来从病毒基因组的特定部分（区）开始DNA复制，而终止似乎没有基因组定义。我们的结论是，起始区和暂停网站的复制程序的主要组织者，但启动，叉进展，并终止复制可以在每个分子。
The duplication of the mammalian genome is an organized event, but there is limited information about the precision of the duplication program at specific genetic loci. We developed an approach that allows DNA replication events to be visualized in individual DNA molecules. Studying the latent replication of Epstein-Barr virus episomes, we show that different initiation sites are used to commence DNA replication from a specific portion of the viral genome (zone), whereas termination does not seem to be genomically defined. We conclude that initiation zones and pausing sites are major organizers of the duplication program, but initiation, fork progression, and termination of replication can vary in each molecule.