DETAILS OF AN ISOLATION METHOD FOR HEPATIC LYMPHOCYTES IN MICE
DETAILS OF AN ISOLATION METHOD FOR HEPATIC LYMPHOCYTES IN MICE
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DOI：
10.1016/0022-1759(92)90223-g
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发表时间：
1992-02-05
影响因子：
2.2
通讯作者：
ABO, T
中科院分区：
文献类型：
--
作者：
WATANABE, H;OHTSUKA, K;ABO, T
肝脏包含独特的淋巴细胞群，即，具有中等强度TcR的胸腺外α-β T细胞。 在本研究中，我们试图确定什么样的预处理是适当的分离肝单核细胞（MNC）含有这样的中间α-β TcR细胞在小鼠。 从未处理的小鼠和出血或肝脏灌注的小鼠中分离肝脏MNC，并鉴定每种制备物中的中间体α-β TcR细胞。 出于简单性、细胞纯度和细胞产量的原因，肝淋巴细胞应从经受完全出血的小鼠中获得。 还提供了通过使用推荐方法获得的胸腺外α-β T细胞的其他信息。
The liver comprises a unique lymphocyte population, i.e., extrathymic alpha-beta T cells with TcR of intermediate intensity. In the present study, we attempted to determine what pretreatments were appropriate to isolate hepatic mononuclear cells (MNC) containing such intermediate alpha-beta TcR cells in mice. Hepatic MNC were isolated from untreated mice and mice subjected to either bleeding or liver perfusion, and the intermediate alpha-beta TcR cells in each preparation were identified. For reasons of simplicity, cell purity and cell yields, hepatic lymphocytes should be obtained from mice subjected to total bleeding. Additional information on extrathymic alpha-beta T cells obtained by using the recommended method is also presented.