Reciprocal control of lncRNA-BCAT1 and β-catenin pathway reveals lncRNA-BCAT1 long non-coding RNA acts as a tumor suppressor in colorectal cancer.
Reciprocal control of lncRNA-BCAT1 and β-catenin pathway reveals lncRNA-BCAT1 long non-coding RNA acts as a tumor suppressor in colorectal cancer.
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lncRNA-BCAT1 和 β-catenin 通路的相互控制揭示了 lncRNA-BCAT1 长非编码 RNA 在结直肠癌中充当肿瘤抑制因子
DOI：
10.18632/oncotarget.15466
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发表时间：
2017-04-04
期刊：
影响因子：
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通讯作者：
Zheng S
中科院分区：
文献类型：
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作者：
Xie F;Xiang X;Huang Q;Ran P;Yuan Y;Li Q;Qi G;Guo X;Xiao C;Zheng S
β-连环蛋白在肿瘤的发生和发展中起重要作用。本研究发现，与邻近的非肿瘤组织相比，β-连环蛋白在30个结直肠癌（CRC）组织样品中上调。使用GSE 18560和GSE 44097数据集（其通过Affytelium plus 2.0微阵列平台生成并从GEO数据库下载）分析长非编码RNA（lncRNA）表达谱，揭示了β-连环蛋白敲低后20种差异表达的lncRNA。我们将其命名为lncRNA-β-catenin associated transcript 1（LncRNA-BCAT 1）。在结直肠癌组织中，lncRNA-BCAT 1表达降低，并且在结直肠癌组织和细胞系中与β-连环蛋白呈负相关。lncRNA-BCAT 1过表达通过下调cyclin D1、c-Myc和MMP-2抑制CRC细胞生长和侵袭。这些结果表明，lncRNA-BCAT 1过表达通过Wnt/β-连环蛋白通路阻断抑制CRC细胞生长和侵袭，并且lncRNA-BCAT 1被Wnt/β-连环蛋白信号转导抑制。这一证据表明，lncRNA-BCAT 1是一种肿瘤抑制因子，lncRNA-BCAT 1可能是CRC中有效的预后生物标志物。
β-catenin plays a major role in tumor development and progression. The present study found that β-catenin was upregulated in 30 samples of colorectal cancer (CRC) tissue as compared to adjacent non-tumor tissues. Analysis of long non-coding RNA (lncRNA) expression profiles using the GSE18560 and GSE44097 datasets, which were generated via the Affymetrix plus 2.0 microarray platform and downloaded from the GEO database, revealed 20 differentially expressed lncRNAs following β-catenin knockdown. We focused on AK091631, a novel lncRNA, which we named lncRNA-β-catenin associated transcript 1 (LncRNA-BCAT1). lncRNA-BCAT1 expression was decreased in CRC tissues, and was negatively associated with β-catenin in both CRC tissues and cell lines. lncRNA-BCAT1 overexpression suppressed CRC cell growth and invasion by downregulating cyclin D1, c-Myc, and MMP-2. These results suggest that lncRNA-BCAT1 overexpression inhibits CRC cell growth and invasion via Wnt/β-catenin pathway blockade, and that lncRNA-BCAT1 is repressed by Wnt/β-catenin signaling. This evidence suggests that lncRNA-BCAT1 is a tumor suppressor and that lncRNA-BCAT1 may be an effective prognostic biomarker in CRC.