Oocyte growth in vitro: potential model for studies of oocyte-granulosa cell interactions.
Oocyte growth in vitro: potential model for studies of oocyte-granulosa cell interactions.
复制标题
卵母细胞体外生长：研究卵母细胞-颗粒细胞相互作用的潜在模型。
DOI：
10.1007/s12522-011-0096-3
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发表时间：
2012
影响因子：
3.4
通讯作者：
Hirao Y
中科院分区：
文献类型：
--
作者：
吉原利一;ほか;西村鷹則・藤井渉・杉浦幸二・内藤邦彦;Hirao Y
促性腺激素、生长因子和类固醇激素等多种因素在哺乳动物卵巢的卵母细胞/卵泡生长调节中起着重要作用。除了这些因素外，卵母细胞和颗粒细胞之间存在双向相互作用，这对于获得最佳的卵母细胞发育能力是必不可少的。卵母细胞通过分泌旁分泌因子来改变邻近卵丘细胞的活动，如特定氨基酸转运体的表达、胆固醇的生物合成和卵丘细胞中糖酵解的水平，在这种相互作用中起着关键作用。在已知的卵母细胞衍生因子中，生长分化因子9(GDF9)是介导卵母细胞调节卵丘扩张和颗粒细胞增殖的主要因子。GDF9经常与其他卵母细胞衍生因子以协同方式相互作用。似乎有理由推测，在体外生长的卵母细胞需要与体内相似的相互作用。一些卵母细胞介导的调控已在体外得到证实，这为培养系统作为此类研究的有力工具提供了证据。本文从卵母细胞-颗粒细胞相互作用的角度讨论了正在生长的卵母细胞的体外培养。
Various factors such as gonadotrophins, growth factors, and steroid hormones play important roles in the regulation of oocyte/follicular growth in mammalian ovaries. In addition to these factors, there is a bidirectional interaction between oocytes and granulosa cells that is essential for achieving optimal oocyte developmental competence. Oocytes play a key role in this interaction by secreting paracrine factors that alter the activities of neighboring cumulus cells, such as the expression of a specific amino acid transporter, cholesterol biosynthesis, and levels of glycolysis in the cumulus cells. Among the known oocyte-derived factors, growth differentiation factor 9 (GDF9) is the dominant factor mediating the regulation by oocytes leading to cumulus expansion and granulosa cell proliferation. GDF9 frequently interacts with other oocyte-derived factors in a synergistic manner. It seems reasonable to speculate that oocytes growing in vitro require interactions similar to those in vivo. Some of the oocyte-mediated regulations have been confirmed in vitro, providing evidence of the usefulness of culture systems as a strong tool for such studies. This review discusses in vitro culture of growing oocytes in terms of oocyte–granulosa cell interactions.