Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization
Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix-assisted laser desorption/ionization
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DOI：
10.1002/jms.153
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发表时间：
2001-04-01
影响因子：
2.3
通讯作者：
Caprioli, RM
中科院分区：
文献类型：
--
作者：
Todd, PJ;Schaaff, TG;Caprioli, RM
有机二次离子质谱（西姆斯）和基质辅助激光解吸/电离（MALDI）质谱可用于产生样品的分子图像。这是通过从平坦样品上的明确识别的点进行电离，并通过在样品表面上移动电离点来执行样品的光栅来实现的。独特的分析能力的质谱映射在组织水平的各种生物样品进行了讨论。西姆斯提供了元素和低分子量化合物的空间分布信息以及这些化合物的分子结构，而MALDI则提供了高分子量化合物的空间信息，包括它们在组织中的极低水平分布，以及这些化合物的分子结构信息。应用这些方法分析问题需要适当的仪器，样品制备方法，和数据表示通常在三坐标图，其中x和y是样品的物理尺寸和z是信号幅度。成像质谱的使用说明了几个生物系统。版权所有（C）2001约翰威利父子有限公司
Organic secondary ion mass spectrometry (SIMS) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry can be used to produce molecular images of samples. This is achieved through ionization from a clearly identified point on a flat sample, and performing a raster of the sample by moving the point of ionization over the sample surface. The unique analytical capabilities of mass spectrometry for mapping a variety of biological samples at the tissue level are discussed. SIMS provides information on the spatial distribution of the elements and low molecular mass compounds as well as molecular structures on these compounds, while MALDI yields spatial information about higher molecular mass compounds, including their distributions in tissues at very low levels, as well as information on the molecular structures of these compounds. Application of these methods to analytical problems requires appropriate instrumentation, sample preparation methodology, and a data presentation usually in a three-coordinate plot where x and y are physical dimensions of the sample and z is the signal amplitude. The use of imaging mass spectrometry is illustrated with several biological systems. Copyright (C) 2001 John Wiley & Sons, Ltd.