XOTCH, THE XENOPUS HOMOLOG OF DROSOPHILA-NOTCH
XOTCH, THE XENOPUS HOMOLOG OF DROSOPHILA-NOTCH
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DOI：
10.1126/science.2402639
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发表时间：
1990-09-21
期刊：
影响因子：
56.9
通讯作者：
KINTNER, C
中科院分区：
文献类型：
--
作者：
COFFMAN, C;HARRIS, W;KINTNER, C
在脊椎动物胚胎的发育过程中，细胞的命运由相邻组织之间传递的感应信号决定。在不了解相关分子机制的情况下，这种决定性的相互作用很难完全描述。果蝇和线虫优雅线虫的突变已经被分离出来，这些突变定义了一系列涉及类似类型的细胞诱导的相关基因产物。其中一个基因，果蝇的Notch基因，与胚芽的神经源性区域、发育中的神经系统和眼睛-触角成像盘的细胞命运选择有关。为了研究脊椎动物胚胎中细胞与细胞之间的相互作用是否也依赖于Notch样分子，我们从带有NotchDNA的爪哇胚胎中分离出互补的DNA克隆。这种方法发现了一种Xenopus分子Xotch，它在结构和发育表达上都与果蝇Nottin非常相似。
During the development of a vertebrate embryo, cell fate is determined by inductive signals passing between neighboring tissues. Such determinative interactions have been difficult to characterize fully without knowledge of the molecular mechanisms involved. Mutations ofDrosophilaand the nematodeCaenorhabditis eleganshave been isolated that define a family of related gene products involved in similar types of cellular inductions. One of these genes, theNotchgene fromDrosophila, is involved with cell fate choices in the neurogenic region of the blastoderm, in the developing nervous system, and in the eye-antennal imaginal disc. Complementary DNA clones were isolated fromXenopusembryos withNotchDNA in order to investigate whether cell-cell interactions in vertebrate embryos also depend onNotch-like molecules. This approach identified aXenopusmolecule,Xotch, which is remarkably similar toDrosophilaNotchin both structure and developmental expression.