Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9
Effect of freeze-thaw cycles on serum measurements of AFP, CEA, CA125 and CA19-9
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DOI：
10.1080/00365510701297480
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发表时间：
2007-01-01
影响因子：
2.1
通讯作者：
Lu, H. -K.
中科院分区：
文献类型：
--
作者：
Gao, Y. -C.;Yuan, Z. -B.;Lu, H. -K.
AFP、CEA、CA 125和CA 19 -9是临床实践中常用的血清肿瘤标志物（TM），尽管其通过免疫测定法的定量可能受到分析前样品处理的影响。尽管反复冻融循环的影响已被普遍认可，但其细节尚不清楚。本研究测量（CLIA）新鲜分离的血清样本中的这些TM，并在5个冻融循环中的每一个循环后进行测量，其中样本在循环4中在-40 ℃下冷冻10个月，在其他循环中冷冻2小时。重复测量的一般线性模型的统计分析显示，四种TM的测量值显著降低，最后一个周期后，CA 125的降低最少，为6.8%，CA 19 -9的降低最多，为18.2%，AFP、CEA和CA 125在第4个周期时的平均单周期显著降低7.7%，对于CA 19 -9，在第4和第5周期分别为7.5%和9.3%。因此，与频繁冻融相比，AFP、CEA和CA 125的测量更容易受到长期冻存的影响，而CA 19 -9在两种条件下都相对不稳定。
AFP, CEA, CA125 and CA19-9 are commonly used serum tumour markers (TMs) in clinical practice, although their quantification by immunoassay may be influenced by pre-analytical sample handling. Though the effect of repetitive freeze-thaw cycles is generally recognized, it is not clear in detail. The present study measured (CLIA) these TMs in serum samples freshly separated and after each of five freeze-thaw cycles, in which the samples were frozen at -40 degrees C for 10 months at cycle 4 and 2 h at other cycles. Statistical analysis with the General Linear Model for Repeated Measures revealed significant decreases in the measurements of the four TMs, with the least decrease of 6.8% for CA125 and the most decrease of 18.2% for CA19-9 after the last cycle, and an overwhelming single cycle decrease of mean 7.7% at cycle 4 for AFP, CEA and CA125, of 7.5% and 9.3% at cycles 4 and 5 for CA19-9. So it seems that measurements of AFP, CEA and CA125 are more readily affected by long-term frozen storage compared with frequent freezing-thawing, while CA19-9 is relatively unstable under both conditions.