Biodegradation kinetic studies for the removal of p-cresol from wastewater using Gliomastix indicus MTCC 3869
Biodegradation kinetic studies for the removal of p-cresol from wastewater using Gliomastix indicus MTCC 3869
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DOI：
10.1016/j.bej.2007.12.015
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发表时间：
2008-06-01
影响因子：
3.9
通讯作者：
Kumar, Arinjay
中科院分区：
文献类型：
--
作者：
Singh, Ravi Kant;Kumar, Shashi;Kumar, Arinjay
研究了新丝状真菌菌株 Gliomastix indicus 的纯培养物对甲酚的生物降解作用。在两个月的时间里，真菌菌株在改良查佩克培养基中适应了更高浓度的对甲酚（700 mg/l）。滞后期为24小时。摇瓶批量实验在 28 摄氏度的温度和 6 的 pH 条件下在较宽的初始对甲酚浓度 (10-700 mg/l) 范围内进行，保持初始生物质浓度恒定在 18.72 mg/l。实验观察到对甲酚是抑制型底物并且对甲酚的抑制作用在浓度高于50mg/l时变得占主导地位。研究了细胞生长和对甲酚降解的动力学。六个动力学模型（Haldane、Andrews、Webb、Yano、Aiba 和 Teissier）适合实验生长动力学数据。 Haldane、Andrews、Webb 和 Yano 的模型（相关系数 R-2 > 0.99）被发现是最合适的模型。此外，还研究了观察到的产量系数Y-x随比生长率mu(g)的变化。维持能量系数和真实生长产量系数分别为0.0227 h(-1)和1.0708 (mg/mg)。比底物利用率以比生物量生长速率表示。发现衰减系数为0.0055 h(-1)。还考察了培养基的pH值和氮源对对甲酚生物降解的影响。 (C) 2007 Elsevier B.V. 保留所有权利。
Biodegradation of p-cresol by a pure culture of new filamentous fungal strain Gliomastix indicus was studied. The fungus strain was acclimatized to the higher concentration of 700 mg/l of p-cresol in modified czapeck medium over a period of two months. The lag period was 24 h. The shake flask batch experiments were carried out at temperature of 28 degrees C and pH of 6 over a wide range of initial p-cresol concentration (10-700 mg/l) keeping initial biomass concentration constant at 18.72 mg/l. Experimentally it was observed that p-cresol was inhibitory type substrate and the inhibition effect of p-cresol became predominant above a concentration of 50 mg/l. The kinetics of cell growth and p-cresol degradation were investigated. Six kinetic models (Haldane, Andrews, Webb, Yano, Aiba, and Teissier) were fitted to the experimental growth kinetic data. Models by Haldane, Andrews, Webb and Yano (correlation coefficient R-2 > 0.99) were found to be most suitable models. In addition, the variation of observed yield coefficient Y-x with specific growth rate mu(g) was investigated. The maintenance energy coefficient and true growth yield coefficient, were found to be 0.0227 h(-1) and 1.0708 (mg/mg), respectively. The specific substrate utilization rate was represented in terms of specific biomass growth rate. The decay coefficient was found to be 0.0055 h(-1). The effect of pH and nitrogen source of medium on biodegradation of p-cresol was investigated as well. (C) 2007 Elsevier B.V. All rights reserved.