Differential activity of recombinant colony-stimulating factors in supporting proliferation of human peripheral blood and bone marrow myeloid progenitors in culture.
Differential activity of recombinant colony-stimulating factors in supporting proliferation of human peripheral blood and bone marrow myeloid progenitors in culture.
复制标题
重组集落刺激因子在支持培养物中人外周血和骨髓祖细胞增殖中的差异活性。
DOI：
10.1111/j.1365-2141.1989.tb07709.x
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发表时间：
1989
影响因子：
6.5
通讯作者：
Rovera,G
中科院分区：
文献类型：
--
作者：
Caracciolo,D;Clark,S;Rovera,G
与骨髓祖细胞不同，从外周血中分离的人髓系祖细胞在存在rhG-CSF、rhM-CSF或rhIL-6的半固体培养基中不形成集落，但在存在rhIL-3或rhGM-CSF的情况下确实形成含有中性粒细胞、巨噬细胞、嗜酸性粒细胞、嗜碱性粒细胞或混合嗜酸性粒细胞-巨噬细胞集落的集落。通过在rhGM-CSF存在下培养血液祖细胞数天来引发血液祖细胞，导致响应于G-CSF和IL-6增殖并形成嗜中性粒细胞集落的细胞频率急剧增加。在IL-3存在下维持的悬浮培养物产生了对GM-CSF和G-CSF有反应的克隆形成细胞数量增加，但对M-CSF或IL-6无反应。rhIL-6不直接刺激外周血祖细胞的集落形成，但确实使它们对G-CSF产生应答。这些结果与粒细胞分化的分级模型一致，其中循环祖细胞通过改变生长因子敏感性的程序按以下顺序依次进行：IL-3、GM-CSF、IL-6和/或G-CSF。
Unlike bone marrow progenitor cells, human myeloid progenitors isolated from peripheral blood do not form colonies in semi‐solid medium in the presence of rhG‐CSF, rhM‐CSF or rhIL‐6, but do form colonies containing neutrophils, macrophages, eosinophils, basophils or mixed neutrophilic‐macrophages colonies in the presence of rhIL‐3 or rhGM‐CSF. Priming of blood progenitors by culturing them for several days in the presence of rhGM‐CSF resulted in a dramatic increase in the frequency of cells that proliferate in response to G‐CSF and IL‐6 and form neutrophilic granulocytic colonies. Suspension cultures maintained in the presence of IL‐3 yielded increased numbers of clonogenic cells responsive to GM‐CSF and G‐CSF, but not to M‐CSF or IL‐6. rhIL‐6 did not directly stimulate colony formation of peripheral blood progenitors but did prime them to respond to G‐CSF. These results are consistent with a hierarchical model of granulocytic differentiation in which circulating progenitors proceed sequentially through a programme of changing growth factor sensitivity with the following sequence: IL‐3, GM‐CSF, IL‐6 and/or G‐CSF.