Geographic distribution of hepatitis B virus (HBV) genotype in patients with chronic HBV infection in Japan
Geographic distribution of hepatitis B virus (HBV) genotype in patients with chronic HBV infection in Japan
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DOI：
10.1053/jhep.2001.27221
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发表时间：
2001-09-01
期刊：
影响因子：
13.5
通讯作者：
Mizokami, M
中科院分区：
文献类型：
--
作者：
Orito, E;Ichida, T;Mizokami, M
日本B型肝炎病毒（HBV）基因型的地理分布及其临床相关性知之甚少。我们研究了731例慢性HBV感染的日本患者。聚合酶链反应（PCR）后用限制性片段长度多态性（RFLP）方法确定HBV基因型。720例PCR阳性患者中，A型12例（1.7%），B型B 88例（12.2%），C型610例（84.7%），D型3例（0.4%），混合型7例（1.0%）。在日本本土，超过94%的患者为C型，而在最南部的岛屿冲绳，60%的患者为C型，在日本本土北方的东北地区，22.9%的患者为B型。与C基因型患者相比，B基因型患者年龄较大（53.6 ~ 42.2岁; P <0.01），B e抗原（HBeAg）阳性率较低（18.4%~ 50.6%; P <0.01），血清HBV DNA水平较低（5.02 ~ 5.87 log基因组当量（LGE）/mL; P <0.01）。基因型B肝癌患者的平均年龄为70.1 ± 9.2岁，而基因型C肝癌患者的平均年龄为55.2 ± 9.7岁（P <0.01）。这些结果表明，在日本，基因型C和B占优势，不同基因型的患者在地理分布和临床特征方面存在显著差异。
The geographic distribution of hepatitis B virus (HBV) genotypes in Japan and its clinical relevance are poorly understood. We studied 731 Japanese patients with chronic HBV infection. HBV genotype was determined by the restriction fragment length polymorphism (RFLP) method after polymerase chain reaction (PCR). Of the 720 patients with positive PCR, 12 (1.7%) were HBV genotype A, 88 (12.2%) were genotype B, 610 (84.7%) were genotype C, 3 (0.4%) were genotype D, and 7 (1.0%) were of mixed genotype. Over 94% of patients on the Japanese mainland had genotype C, while 60% of the patients on Okinawa, the most southern islands, and 22.9% in the Tohoku area, the northern part of the mainland, harbored genotype B. Compared with genotype C patients, genotype B patients were older (53.6 to 42.2 years; P < .01), had a lower rate of positive hepatitis B e antigen (HBeAg) (18.4% to 50.6%; P < .01), and a lower level of serum HBV DNA (5.02 to 5.87 log genome equivalents (LGE)/mL; P < .01). The mean age of the genotype B patients with hepatocellular carcinoma was 70.1 +/- 9.2 years, compared with 55.2 +/- 9.7 of genotype C patients (P < .01). These results indicate that genotypes C and B are predominant in Japan, and there are significant differences in geographic distribution and clinical characteristics among the patients with the different genotypes.