On-DemandAdhesive Control ofFunctional Gel-Tool forOn-ChipCellMeasurement
On-DemandAdhesive Control ofFunctional Gel-Tool forOn-ChipCellMeasurement
复制标题
用于片上细胞测量的功能性凝胶工具的按需粘合剂控制
DOI：
--
复制
发表时间：
2008
期刊：
影响因子：
--
通讯作者：
T. Fukuda
中科院分区：
文献类型：
--
作者：
H. Maruyama;T. Fukuda
近年来，针对环境条件的按需粘合剂控制功能凝胶工具的分析得到了发展[6,7]。开发了片上细胞实验。由于不能使用微操作器进行浓度控制，因此采用紫外照明控制凝胶工具进行芯片细胞实验的许多技术都是在可调节电解质溶液中进行的。凝胶工具粘附在封闭空间的物体上，如玻璃微芯片。几种力，如基片，其他凝胶工具和电池与目标接触的电力，磁力，水动力和紫外线照射下的物体。在没有紫外线照射的情况下，使用光学镊子进行细胞操作。在大多数情况下，凝胶工具不能在相同的电泳和介电区粘附对象。凝胶工具是由亲水性著名方法的盐析制成的[8,9]。虽然这些方法都适合光交联树脂。这种凝胶工具可以进行顺序和连续操作，随机和功能化，通过引入功能材料，这样的选择性操作是困难的。此外，作为指标的印象。我们生产的凝胶工具浸渍了高压，可能会对电池造成一定的损坏。磁性pH指示剂溴百里酚蓝（BTB）被认为对细胞是安全的，在中性范围内对细胞是失效的，用于测量环境pH值分离[10,11]。然而，靶细胞对细胞的修饰。传感凝胶工具是由磁珠操纵的，因为大多数光学镊子都需要操作。ph值是通过检测没有磁性的细胞来测量的。流体动力学操作是凝胶工具校准颜色信息的颜色。我们成功地测量了单个外部泵阀bb0周围的局部ph值。光学镊子通过在微芯片上对单个目标进行环绕细胞、环形三维操纵来实现对细胞的控制。然而，光镊可能会对ph传感凝胶工具造成细胞损伤。
analysis, whichisrobust against thedisturbance ofthe Anon-demand adhesive control offunctional gel-tool for environment conditions, isdeveloped inthese days[6,7]. on-chip cell experiment wasdeveloped. Adhesiveness of A lotoftechniques fortheon-chip cell experiment with thegel-tool wascontrolled byUV illumination inan cell manipulation andcell immobilization aredeveloped electrolyte solution of an adjustedelectrolyte because micromanipulator can'tbe employed inthe concentration. Gel-tool adheres toobjects suchasglass microchip whichisclosed space. Several forces suchas substrate, other gel-tool andcell bycontact tothetarget electric force, magnetic force, hydrodynamic force, and object underUV illumination. Without UV illumination, optical tweezers areemployed forcellmanipulation. gel-tool doesnotadheretoobjects inthesame Electrophoresis anddielectrophoresis areoneofthemost conditions. Gel-tool ismadebysalting-out ofhydrophilicfamous methods [8,9].Although these methods aresuitable photo-crosslinkable resin.Thisgel-tool can be forsequential andcontinuous manipulation, randomand functionalized byintroducing functional materials such selective manipulation isdifficult. Moreover, impress of asindicators. We produced gel-tool impregnated with highvoltage maycausesomedamagetocell. Magnetic pH indicator Bromothymol blue(BTB), whichisused force isconsidered tobesafe tocell andisemployed tocell forneutral range, formeasurement ofambient pHvalue separation [10, 11].However, modification ofcell bythe oftarget cell. ThepHsensing gel-tool ismanipulated by magnetic beadformanipulation isrequired because most optical tweezers. ThepHvalue ismeasured bydetectingcells don't havemagnetism. Hydrodynamic manipulation is thecolor ofthegel-tool incalibrated color information. difficult for2-Dor3-Dmanipulations ofcellwithan We succeeded inlocal pHmeasurement aroundasingle external pumpandvalve [12]. Optical tweezers canachieve yeastcellbysurrounding thecellusingring-shaped3-Dmanipulation ofasingle target inamicrochip [13]. gel-tool andbyimmobilizing thecell onthesurface of However, optical tweezers maycause damage tothecell by thepHsensing gel-tool.
影响因子：
7.4
作者：
Kuhara, M;Takeyama, H;Matsunaga, T
通讯作者：
Matsunaga, T