HMB-45 immunohistochemical staining of conjunctival melanocytic lesions.
HMB-45 immunohistochemical staining of conjunctival melanocytic lesions.
复制标题
结膜黑素细胞病变的 HMB-45 免疫组织化学染色。
DOI：
10.1016/s0161-6420(91)32281-4
复制
发表时间：
1991
期刊：
影响因子：
13.7
通讯作者：
Wagner,D
中科院分区：
文献类型：
--
作者：
McDonnell,JM;Sun,YY;Wagner,D
HMB-45是一种单克隆抗体，最近被描述为对皮肤的黑色素细胞增殖和黑色素病变的转移具有高度特异性。为了确定HMB-45在眼科病理学中的可能作用，使用免疫组织化学技术以抗S-100和HMB-45作为一抗分析了45个结膜病变，包括23个黑素瘤。19例（82.6%）黑色素瘤显示HMB-45阳性，19例（82.6%）含有S-100蛋白，除2例外，其余均一致。研究了7例原发性获得性黑变病：3例有黑色素沉着的患者中有1例（33%）HMB-45阳性，4例无黑色素沉着的患者中有2例（50%）HMB-45阳性。在痣中，1/11例（9.1%）显示HMB-45微弱染色。15例结膜上皮发育不良与HMB-45呈阴性。目前，HMB-45在结膜黑色素瘤的分析中似乎没有提供比S-100蛋白更大的优势。其在区分良性与非典型或恶性交界性黑素细胞增生中的作用尚不清楚。
HMB-45 is a monoclonal antibody recently described as being highly specific for melanocytic proliferations of the skin and in metastases of melanotic lesions. To determine a possible role for HMB-45 in ophthalmic pathology, 45 conjunctival lesions, including 23 melanomas, were analyzed using immunohistochemical techniques with anti-S-100 and HMB-45 as primary antibodies. Nineteen (82.6%) of the melanomas exhibited HMB-45 positivity, and 19 (82.6%) contained S-100 protein, with concordance of all but two cases. Seven cases of primary acquired melanosis were studied: one (33%) of three with atypia was HMB-45 positive, as were two (50%) of four without atypia. Among nevi, 1(9.1%) of 11 showed faint staining with HMB-45. Fifteen conjunctival epithelial dysplasias were negative with HMB-45. At present, HMB-45 appears to offer no great advantage over S-100 protein in the analysis of conjunctival melanomas. Its role in the distinction of benign from atypical or malignant junctional melanocytic proliferations remains unclear.