Chromosomal aberrations in colorectal cancers and liver metastases analyzed by comparative genomic hybridization
Chromosomal aberrations in colorectal cancers and liver metastases analyzed by comparative genomic hybridization
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DOI：
10.1002/ijc.1522
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发表时间：
2001-12-01
影响因子：
6.4
通讯作者：
Yamagishi, H
中科院分区：
文献类型：
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作者：
Aragane, H;Sakakura, C;Yamagishi, H
比较基因组杂交（CGH）用于筛选30例原发性结直肠癌和16例肝转移瘤中DNA序列拷贝数的变化，以确定包含对结直肠癌发生和进展重要的基因的区域。在原发性结直肠癌中，我们发现在7 p21（36.7%）、7q31-36（30%）、8q23-24（43.0%）、12 p（30%）、14 q24 -32（33.3%）、16 p（40.0%）、20 p（33.3%）、20 q（63.3%）和21 q（36.3%），而18 q12 -23（36.7%）常出现缺失。在转移性肿瘤中，与原发性肿瘤相比，有更多的DNA序列获得和丢失，在8 q23 -24（发现于62.5%的复发肿瘤和43.0%的原发肿瘤），15 q21 -26（37.5% vs. 20.0%），19 p（43.8% vs. 20.0%）和20 q（81.3% vs. 63.3%）以及l 8 q12 -23丢失（50.0% vs. 36.7%）。在转移性肿瘤中观察到的遗传变化模式，在8 q23 -24和20 q频繁增加，在18 q12 -23丢失，表明结直肠癌的进展。我们对所有接受CGH检查的患者进行了临床随访研究，并将我们的注意力集中在8 q和20 q的遗传变化上。具有这些遗传改变的原发性结直肠癌患者的肝转移发生率较高。在8 q和20 q的增益可能是有用的，以确定患者在发展肝转移的高风险。(C)2001 Wiley-Liss，Inc.
Comparative genomic hybridization (CGH) was used to screen for changes in the number of DNA sequence copies in 30 primary colorectal cancers and 16 liver metastases, to identify regions that contain genes important for the development and progression of colorectal cancer. In primary colorectal cancer, we found frequent gains at 7p21 (36.7%), 7q31-36 (30%), 8q23-24 (43.0%), 12p (30%), 14q24-32 (33.3%), 16p (40.0%), 20p (33.3%), 20q (63.3%) and 21q (36.3%), while loss was often noted at 18q12-23 (36.7%). In metastatic tumors, there were significantly more gains and losses of DNA sequences than in primary tumors, with gains at 8q23-24 (found in 62.5% of recurrences vs. 43.0% of primary tumors), 15q21-26 (37.5% vs. 20.0%), 19p (43.8% vs. 20.0%) and 20q (81.3% vs. 63.3%) and losses at l8q12-23 (50.0% vs. 36.7%). The pattern of genetic changes seen in metastatic tumors, with frequent gains at 8q23-24 and 20q and loss at 18q12-23, suggests the progression of colorectal cancer. We investigated a clinical follow-up study for all patients examined by CGH and directed our attention to the genetic changes consisting of gains at 8q and 20q. The incidence of liver metastases was higher in patients with primary colorectal cancer with these genetic changes. Gains at 8q and 20q might be useful to identify patients at high risk for developing liver metastases. (C) 2001 Wiley-Liss, Inc.