Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera)
Characterization and influences of classical insect hormones on the expression profiles of a molting carboxypeptidase A from the cotton bollworm (Helicoverpa armigera)
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DOI：
10.1111/j.1365-2583.2009.00879.x
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发表时间：
2009-06
影响因子：
2.6
通讯作者：
Yi-Peng Sui;X. Liu;Lian‐Qin Chai;J‐X. Wang;X‐F. Zhao
中科院分区：
文献类型：
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作者：
Yi-Peng Sui;X. Liu;Lian‐Qin Chai;J‐X. Wang;X‐F. Zhao
蜕皮是节肢动物非常重要的生理行为。在蜕皮过程中，发生珠被解体，这是旧的内表皮的消化和吸收，以形成新的角质层。蛋白酶在此过程中发挥着关键作用。蜕皮羧肽酶 A (Ha-CPA) 是棉铃虫的特征。 Ha-CPA 转录本主要存在于 5 龄幼虫的珠被中。在珠被中，该基因的转录水平分别在第5龄蜕皮期和第6龄预蛹期达到峰值。免疫组织化学检查表明，Ha-CPA可以分布到蜕皮期和蛹前期幼虫的蜕皮液中。 Ha-CPA 的表达可以被 20-羟基蜕皮酮 (20E) 上调。这些事实表明Ha-CPA参与了幼虫蜕皮和变态过程中体被的解离。
Molting is a very important physiological behavior to arthropods. During molting, integument apolysis occurs, which is the digestion and absorption of the old endocuticle for new cuticle formation. Proteases play critical roles in this process. Molting carboxypeptidase A (Ha‐CPA) is characterized from Helicoverpa armigera. The Ha‐CPA transcript was mainly present in the integument from the 5th instar larvae. In the integument, the transcription level of the gene reached its peak at the 5th instar molting stage and the 6th instar prepupal stage, respectively. The examination of immunohistochemistry revealed that Ha‐CPA could distribute into the molting fluid in the molting‐ and prepupal‐stage larvae. The expression of Ha‐CPA could be up‐regulated by 20‐hydroxyecdysone (20E). These facts indicate that Ha‐CPA participates in the apolysis of the integument during larval molting and metamorphosis.