CROSS-LINKS BETWEEN STEREOCILIA IN THE GUINEA-PIG COCHLEA
CROSS-LINKS BETWEEN STEREOCILIA IN THE GUINEA-PIG COCHLEA
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DOI：
10.1016/0378-5955(85)90010-3
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发表时间：
1985-01-01
期刊：
影响因子：
2.8
通讯作者：
HACKNEY, CM
中科院分区：
文献类型：
--
作者：
FURNESS, DN;HACKNEY, CM
豚鼠耳蜗毛细胞静纤毛之间的交联已被检查使用高分辨率扫描（SEM）和透射电子显微镜（TEM），证实了这些结构的最新描述。在内毛细胞和外毛细胞上，观察到从较短静纤毛的尖端到相邻较高静纤毛的侧部的链接（向上指向链接）、同一行静纤毛之间的链接（侧到侧链接）和相邻行之间的链接（行到行链接）。在使用（1）仅戊二醛、（2）戊二醛/锇和（3）戊二醛/锇/硫代碳酰肼（一种无需镀金的技术）固定的材料中观察到这些连接。与固定（1）和（2）相比，固定（3）后的静纤毛和微绒毛表面更光滑，而固定（1）和（2）后的静纤毛和微绒毛表面通常粗糙。在TEM中，侧到侧和行到行的链接在静纤毛之间形成规则的晶格，并且由许多股组成。向上指向的链接由一条单链组成，其末端与电子密度高的物质相关联。这位于静纤毛膜和肌动蛋白丝束之间，在较短静纤毛的尖端和较高静纤毛的侧面。
Cross-links between stereocilia on guinea pig cochlear hair cells have been examined using high resolution scanning (SEM) and transmission electron microscopy (TEM), confirming recent descriptions of these structures. Links from the tips of shorter stereocilia to the sides of the adjacent taller stereocilia (upward-pointing links), between stereocilia of the same row (side-to-side links) and between adjacent rows (row-to-row links), have been observed on inner and outer hair cells. These links have been seen in material fixed using (1) glutaraldehyde only, (2), glutaraldehyde/osmium and (3) glutaraldehyde/osmium/thioarbohydrazide (a technique which makes gold coating unnecessary). Upward-pointing links were seen less frequently, and the surfaces of stereocilia and microvilli were smoother after fixation (3) compared with fixations (1) and (2) in which they were usually roughened in appearance. In TEM, side-to-side and row-to-row links form a regular lattice between stereocilia, and consist of a number of strands. Upward-pointing links consist of a single strand, the ends of which are associated with electron-dense material. This lies between the stereociliary membrane and the actin filament bundle, at the tip of the shorter stereocilium and the side of the taller stereocilium.