Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.
Abnormalities in the basement membrane structure promote basal keratinocytes in the epidermis of hypertrophic scars to adopt a proliferative phenotype.
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基底膜结构的异常促进肥厚性疤痕表皮中的基底角质形成细胞采取增殖表型
DOI：
10.3892/ijmm.2016.2519
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发表时间：
2016-05
影响因子：
5.4
通讯作者：
Fu X
中科院分区：
文献类型：
--
作者：
Yang S;Sun Y;Geng Z;Ma K;Sun X;Fu X
大多数关于疤痕形成的研究主要集中在真皮，而对表皮的参与知之甚少。此前的研究表明，疤痕组织来源的角质形成细胞在遗传和细胞生物学水平上都与正常细胞不同。然而，在疤痕形成过程中导致角质形成细胞基本异常的机制仍然难以捉摸。为此，在本研究中，我们使用正常、伤口边缘和肥厚性疤痕组织来检查表皮再生过程中发生的形态变化，作为伤口愈合过程的一部分，发现肥厚性疤痕组织的组织学结构与正常皮肤不同，表皮厚度显着增加。值得注意的是，疤痕组织中似乎不存在基底膜（BM）染色。此外，细胞角蛋白（CK）10、CK14、CK5、CK19和整合素-β1的免疫荧光染色表明，正常组织、伤口边缘和肥厚性疤痕组织的表皮角质形成细胞中细胞标志物的表达存在差异，这与BM结构的改变相对应。通过使用一组与 BM 成分相关的蛋白质，我们验证了我们的假设，即 BM 在皮肤伤口愈合过程中调节表皮角质形成细胞的细胞命运决定中发挥着重要作用。 BM 结构的改变促进基底角质形成细胞在体内和体外采用增殖表型。
The majority of studies on scar formation have mainly focused on the dermis and little is known of the involvement of the epidermis. Previous research has demonstrated that the scar tissue-derived keratinocytes are different from normal cells at both the genetic and cell biological levels; however, the mechanisms responsible for the fundamental abnormalities in keratinocytes during scar development remain elusive. For this purpose, in this study, we used normal, wound edge and hypertrophic scar tissue to examine the morphological changes which occur during epidermal regeneration as part of the wound healing process and found that the histological structure of hypertrophic scar tissues differed from that of normal skin, with a significant increase in epidermal thickness. Notably, staining of the basement membrane (BM) appeared to be absent in the scar tissues. Moreover, immunofluorescence staining for cytokeratin (CK)10, CK14, CK5, CK19 and integrin-β1 indicated the differential expression of cell markers in the epidermal keratinocytes among the normal, wound edge and hypertrophic scar tissues, which corresponded with the altered BM structures. By using a panel of proteins associated with BM components, we validated our hypothesis that the BM plays a significant role in regulating the cell fate decision of epidermal keratinocytes during skin wound healing. Alterations in the structure of the BM promote basal keratinocytes to adopt a proliferative phenotype both in vivo and in vitro.