Proteomic of lipid rafts in the exocrine pancreas from diet-induced obese rats
Proteomic of lipid rafts in the exocrine pancreas from diet-induced obese rats
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DOI：
10.1016/j.bbrc.2007.02.037
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发表时间：
2007-04-13
影响因子：
3.1
通讯作者：
Hiol, Abel
中科院分区：
文献类型：
--
作者：
Berkane, Amine Adda;Nguyen, Hang Thi Thu;Hiol, Abel
在本研究中，我们采用高能量淀粉饮食诱导大鼠肥胖，并研究了外分泌胰腺的反应。采用外分泌胰腺的酶原颗粒（ZG）或纯化质膜（PM）分离耐洗涤剂膜（DRMs）。基于高含量的胆固醇、GM1、胆汁盐依赖性脂肪酶（BSDL）和GP2富集，低密度馏分被定义为脂筏。同时用抗BSDL免疫金标记法测定木筏的囊泡。通过MALDI-TOF/MS和纳米lc ESI - Q-TOF MS/MS结合鉴定，我们从脂质膜中筛选出33个蛋白，并将其划分为至少4个功能家族。我们的数据表明，饮食诱导的肥胖大鼠的腺泡PM可能被组织成脂质筏，而脂质筏蛋白质组的表征有助于提高我们对肥胖下食物消化的理解。(c) 2007爱思唯尔公司版权所有。
In the present work, we induced obesity in rats with high-energy-starch diet and Studied exocrine pancreas response. The zymogen granule (ZG) or purified plasma membrane (PM) from the exocrine pancreas was used for the isolation of the detergent-resistant membranes (DRMs). Based on high content of cholesterol, GM1, the bile salt dependent lipase (BSDL), and GP2 enrichment, the low-density fractions were defined as lipid rafts. Additionally, the rafts vesicles were determined by immunogold labeling with anti BSDL. By combining MALDI-TOF/MS and nano-LC ESI Q-TOF MS/MS proteoinic identification we have selected 33 proteins from the lipid rafts which were classified into at least four functional families. Our data suggest that the acinar PM from the diet-induced obesity rats may be organized into lipid rafts, and characterization of rafts proteome can contribute to improve our understanding of food digestion under obesity. (c) 2007 Elsevier Inc. All rights reserved.