FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM
FACTORS AFFECTING GROWTH OF STAPHYLOCOCCUS AUREUS L FORMS ON SEMIDEFINED MEDIUM
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DOI：
10.1128/jb.87.5.1192-1197.1964
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发表时间：
1964-01-01
影响因子：
3.2
通讯作者：
BANVILLE, RR
中科院分区：
文献类型：
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作者：
BANVILLE, RR
发现半限定琼脂培养基适合生产和培养 L 型金黄色葡萄球菌。在半限定液体培养基中，L型以含有大量细胞的沉积物的形式生长，但在含有0.05%琼脂的相同培养基中发生大量且弥散的生长。在固体培养基上形成 L 菌落的最佳 pH 值为 6.5。在 0.75% 琼脂上形成的 L 菌落多于在较高浓度琼脂下形成的 L 菌落。 L-菌落在倾倒板上比在划线板上发育得更多，并且在某些情况下，在厌氧培养下出现更多的L-菌落。 L-集落形成似乎被柠檬酸钠抑制。所研究的 L 型的维生素需求量与经典型相似。
A semidefined agar medium was found suitable for production and cultivation of the L-form of Staphylococcus aureus. In semidefined liquid medium, growth of the L-form took place in the form of a sediment containing large masses of cells, but heavy and diffuse growth occurred in the same medium with 0.05% agar. The optimal pH for L-colony formation on solid medium was 6.5. More L-colonies developed on 0.75% agar than at higher agar concentrations. L-colonies developed in greater numbers on pour plates than on streak plates, and in some cases more L-colonies appeared under anaerobic incubation. L-colony formation appeared to be inhibited by sodium-citrate. The vitamin requirements of the L-forms studied were similar to those of the classical form.