Aging influences multiple incidices of oxidative stress in the aortic media of the Fischer 344/NNiaxBrown Norway/BiNia rat.
Aging influences multiple incidices of oxidative stress in the aortic media of the Fischer 344/NNiaxBrown Norway/BiNia rat.
复制标题
衰老影响 Fischer 344/NNiaxBrown 挪威/BiNia 大鼠主动脉中层氧化应激的多种发生。
DOI：
10.1080/10715760500464957
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发表时间：
2006
影响因子：
3.3
通讯作者：
Blough,ER
中科院分区：
文献类型：
--
作者：
Rice,KM;Preston,DL;Walker,EM;Blough,ER
在此，我们确定了衰老对成年（6个月）、老龄（30个月）和极老龄（36个月）Fischer 344/NNiaHSd× Brown Norway/BiNia（F344/N× BN）大鼠主动脉中多种氧化应激标志物的影响。与成年人相比，在36个月的妊娠期，通过氢乙啶（HE）氧化为乙啶（Et）测定的增加了79.7±7.0%，这一发现与奖牌厚度（r= 0.773，p< 0.01）和总蛋白硝化（r= 0.706，p< 0.01）的增加高度相关，但与细胞增殖标志物Ki 67无关。回归分析显示，随着年龄的增长，主动脉超氧阴离子（）的增加与参与代谢（AMPK-α）、信号传导（丝裂原活化蛋白激酶（MAPK）沿着c-Src）、凋亡（Bax、Bcl-2、Traf-2）和转录（NF-κB）活性的蛋白表达和/或调节的变化显著相关。这些结果表明，老化的F344/N× BN主动脉可能非常适合于揭示导致主动脉氧化应激年龄相关变化的分子事件。
Here, we determine the influence of aging on multiple markers of oxidative stress in the aorta of adult (6-month), aged (30-month) and very aged (36-month) Fischer 344/NNiaHSd× Brown Norway/BiNia (F344/N× BN) rats. Compared to adults, increases in as determined by oxidation of hydroethidine (HE) to ethidium (Et) were increased 79.7±7.0% in 36-month aortae and this finding was highly correlated with increases in medal thickness (r= 0.773, p< 0.01) and total protein nitration (r= 0.706, p< 0.01) but not Ki67, a marker for cell proliferation. Regression analysis showed that increases in aortic superoxide anion () with aging were significantly correlated with changes in the expression and/or regulation of proteins involved in metabolic (AMPK-α), signaling (mitogen activated protein kinases (MAPKs) along with c-Src), apoptotic (Bax, Bcl-2, Traf-2) and transcriptional (NF-κB) activities. These results suggest that the aging F344/N× BN aorta may be highly suited for unraveling the molecular events that lead to age-associated alterations in aortic oxidative stress.