1,25-DIHYDROXYVITAMIN D-3 SUPPRESSES CELL-GROWTH, DNA-SYNTHESIS, AND PHOSPHORYLATION OF RETINOBLASTOMA PROTEIN IN A BREAST-CANCER CELL-LINE
1,25-DIHYDROXYVITAMIN D-3 SUPPRESSES CELL-GROWTH, DNA-SYNTHESIS, AND PHOSPHORYLATION OF RETINOBLASTOMA PROTEIN IN A BREAST-CANCER CELL-LINE
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DOI：
10.3109/07357909509094463
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发表时间：
1995-01-01
影响因子：
2.4
通讯作者：
YU, WCY
中科院分区：
文献类型：
--
作者：
FAN, FS;YU, WCY
发现乳腺癌细胞系CAMA-IEe的细胞含有中等浓度的1，25-二羟基维生素D-3 [1，25（OH）（2）D-3]受体。Scatchard分析计算受体的解离常数为4.1 × 10 ~（-10）。这些受体在线性5-20%蔗糖密度梯度上沉积在约4.0S位置，并表现出约46 kDa的表观分子量。受体浓度被认为是最高的细胞生长的早期阶段，不断下降时，细胞生长活跃，并达到最低水平的融合。观察到浓度约为10（-7）M的1，25（OH）（2）D-3抑制细胞生长、DNA合成和视网膜母细胞瘤蛋白磷酸化。用10（-7）M 1，25（OH）（2）D-3处理12小时或更长时间可引起CAMA-IEe细胞胞浆游离钙升高。这些结果表明，CAMA-IEe细胞生长受到1，25（OH）（2）D-3的抑制，这种抑制可能是通过钙动员介导的。
Cells of a breast adenocarcinoma cell line, CAMA-IEe, were found to contain a moderate concentration of 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3] receptors. Thee receptors' dissociation constant calculated by Scatchard analysis was 4.1 x 10(-10). These receptors sedimented at around 4.0 S position on a linear 5-20% sucrose density gradient and exhibited an apparent molecular weight of about 46 kDa. Receptor concentration was found to be highest in the early stages of cell growth, decreased continuously when cells were actively growing, and reached the lowest level at confluence. Suppression of cell growth, of DNA synthesis, and of the phosphorylation of retinoblastoma protein by 1,25(OH)(2)D-3 at concentrations about 10(-7) M were observed. Treatment with 10(-7) M 1,25(OH)(2)D-3 for 12 hr or longer caused elevation of cytosolic free calcium in CAMA-IEe cells. These results show that CAMA-IEe cell growth is suppressed by 1,25(OH)(2)D-3 and that the suppression is possibly mediated through calcium mobilization.