Molecular evolution of voltage-sensitive ion channel genes: on the origins of electrical excitability.
Molecular evolution of voltage-sensitive ion channel genes: on the origins of electrical excitability.
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DOI：
10.1093/oxfordjournals.molbev.a039986
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发表时间：
1993
影响因子：
10.7
通讯作者：
M. Strong;K. Chandy;George A. Gutman
中科院分区：
文献类型：
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作者：
M. Strong;K. Chandy;George A. Gutman
我们分析了各种电压敏感离子通道的核酸和氨基酸序列比对，使用几种方法进行系统发育树重建。这个家族中的古代复制产生了三个远亲群体，一个由Na+和Ca++通道组成，另一个由K+通道组成，第三个包括环核苷酸结合通道。一系列基因复制产生了至少7种与果蝇Shaker K+通道同源的哺乳动物；在所有被研究的三种哺乳动物物种（小鼠、大鼠和人类）中，只有三种基因的克隆是可用的，这表明在这些物种中的一个或另一个物种中还没有恢复特定的基因。与果蝇相比，shaw相关的K+通道和Na+通道家族在哺乳动物中也经历了相当大的扩张。这些扩张大概反映了哺乳动物高度生理和神经复杂性的需要。对四畴通道（Ca++和Na+）的分离域的分析支持它们通过两次连续的基因复制进化而来，并暗示历史上存在一个功能性的两畴通道。
We have analyzed nucleic acid and amino acid sequence alignments of a variety of voltage-sensitive ion channels, using several methods for phylogenetic tree reconstruction. Ancient duplications within this family gave rise to three distantly related groups, one consisting of the Na+ and Ca++ channels, another the K+ channels, and a third including the cyclic nucleotide-binding channels. A series of gene duplications produced at least seven mammalian homologues of the Drosophila Shaker K+ channel; clones of only three of these genes are available from all three mammalian species examined (mouse, rat, and human), pointing to specific genes that have yet to be recovered in one or another of these species. The Shaw-related K+ channels and the Na+ channel family have also undergone considerable expansion in mammals, relative to flies. These expansions presumably reflect the needs of the high degree of physiological and neuronal complexity of mammals. Analysis of the separate domains of the four-domain channels (Ca++ and Na+) supports their having evolved by two sequential gene duplications and implies the historical existence of a functional two-domain channel.