Thermal considerations for microswimmer trap-and-release using standing surface acoustic waves
Thermal considerations for microswimmer trap-and-release using standing surface acoustic waves
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DOI：
10.1039/d1lc00257k
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发表时间：
2021-05-07
期刊：
影响因子：
6.1
通讯作者：
Meacham, J. Mark
中科院分区：
文献类型：
--
作者：
Cui, Mingyang;Kim, Minji;Meacham, J. Mark
由于声学限制的无标记和无接触性质，使用基底声波（SAW;常规地称为表面声波）的细胞和微生物的受控捕获已被证明在许多生物学和生物医学应用中是有用的。然而，由于振动阻尼和其他系统损失而产生的过度加热可能会损害SAW技术的生物相容性。在这里，我们调查的热生物相容性的聚二甲基硅氧烷（PDMS）为基础的SAW和玻璃为基础的SAW [支持体声波（BAW）在流体域]设备在不同的频率和施加的电压下工作。首先，我们使用红外热成像技术来产生PDMS和玻璃基器件的SAW换能器孔径内的感兴趣区域（ROI）的热图。然后使用运动的莱茵衣藻细胞来测试这些装置的捕获性能和生物相容性。在低输入功率下，基于PDMS的SAW系统无法产生足够大的声学捕获力来保持游动C。reinhardtii细胞在高输入功率下，该设备的温度迅速上升，破坏（并可能杀死）细胞。另一方面，玻璃基SAW/BAW混合系统不仅可以捕获游泳的C。在低输入功率下，与基于PDMS的SAW系统相比，Reinhardtii具有更好的热生物相容性，但在高输入功率下也表现出更好的热生物相容性。因此，基于玻璃的SAW/BAW设备在生物相容性环境中产生强大的声学捕获力，为涉及运动细胞和微生物的研究提供了安全捕获活性微泳者的新解决方案。
Controlled trapping of cells and microorganisms using substrate acoustic waves (SAWs; conventionally termed surface acoustic waves) has proven useful in numerous biological and biomedical applications owing to the label- and contact-free nature of acoustic confinement. However, excessive heating due to vibration damping and other system losses potentially compromises the biocompatibility of the SAW technique. Herein, we investigate the thermal biocompatibility of polydimethylsiloxane (PDMS)-based SAW and glass-based SAW [that supports a bulk acoustic wave (BAW) in the fluid domain] devices operating at different frequencies and applied voltages. First, we use infrared thermography to produce heat maps of regions of interest (ROI) within the aperture of the SAW transducers for PDMS- and glass-based devices. Motile Chlamydomonas reinhardtii algae cells are then used to test the trapping performance and biocompatibility of these devices. At low input power, the PDMS-based SAW system cannot generate a large enough acoustic trapping force to hold swimming C. reinhardtii cells. At high input power, the temperature of this device rises rapidly, damaging (and possibly killing) the cells. The glass-based SAW/BAW hybrid system, on the other hand, can not only trap swimming C. reinhardtii at low input power, but also exhibits better thermal biocompatibility than the PDMS-based SAW system at high input power. Thus, a glass-based SAW/BAW device creates strong acoustic trapping forces in a biocompatible environment, providing a new solution to safely trap active microswimmers for research involving motile cells and microorganisms.