Spatial representation of hydrocarbon odorants in the ventrolateral zones of the rat olfactory bulb
Spatial representation of hydrocarbon odorants in the ventrolateral zones of the rat olfactory bulb
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DOI：
10.1152/jn.00873.2004
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发表时间：
2005-02-01
影响因子：
2.5
通讯作者：
Mori, K
中科院分区：
文献类型：
--
作者：
Igarashi, KM;Mori, K
嗅球（OB）的肾小球薄片形成气味受体分布图，这些分布图被包裹成不同的区域。我们之前报道了OB背侧区（1区）单个肾小球的分子接受范围（MRR）特性，并表明极性官能团在该区域的肾小球激活中起主要作用。然而，肾小球2-4区的MRR特性尚不清楚。利用本质信号成像的方法，我们记录了气味剂诱导的OB大鼠腹外表面（2-4区）肾小球的活动。虽然缺乏极性官能团的碳氢化合物气味剂只激活了1区的少数肾小球，但我们发现一系列碳氢化合物气味剂持续激活了腹外表面的许多肾小球。对烃类反应的肾小球分为两组；一个簇（簇H）的肾小球对苯族烃有反应，但对环萜烯烃没有反应。另一个簇（簇I）的肾小球对这两类碳氢化合物都有反应。用碳氢化合物气味剂和含极性官能团气味剂对单个肾小球的MRR特性进行详细分析表明，H和I簇中有效激活肾小球的共同和特有的分子特征是碳氢化合物骨架。这些结果表明，这些集群中以肾小球为代表的嗅觉器官主要识别气味分子的碳氢化合物骨架，因此暗示了1区和2-4区的嗅觉器官在识别气味分子特征的方式上存在系统差异。
The glomerular sheet of the olfactory bulb (OB) forms odorant receptor maps that are parceled into zones. We previously reported the molecular receptive range (MRR) property of individual glomeruli in the dorsal zone (zone 1) of the OB and showed that polar functional groups play a major role in activating glomeruli in this zone. However, the MRR property of glomeruli in zones 2-4 is not well understood yet. Using the method of intrinsic signal imaging, we recorded odorant-induced glomerular activity from the ventrolateral surface (zones 2-4) of rat OB. While hydrocarbon odorants that lack polar functional groups activate only a few glomeruli in zone 1, we found that a series of hydrocarbon odorants consistently activated many glomeruli in the ventrolateral surface. The hydrocarbon-responsive glomeruli were grouped into two clusters; glomeruli in one cluster (cluster H) responded to benzene-family hydrocarbons but not to cyclic terpene hydrocarbons. Glomeruli in the other cluster (cluster I) responded to both classes of hydrocarbons. Detailed analyses of MRR properties of individual glomeruli using hydrocarbon odorants and polar-functional-group-containing odorants showed that the common and characteristic molecular features effective in activating glomeruli in the clusters H and I are the hydrocarbon skeleton. These results suggest that ORs represented by glomeruli in these clusters recognize primarily the hydrocarbon skeleton of odorants, and thus imply a systematic difference in the manner of recognizing odorant molecular features between ORs in zone 1 and ORs in zones 2-4.