The conserved MASRPF motif in the Attractin homolog, Distracted, is required for association with Drosophila E3-ligase Mgrn1.
The conserved MASRPF motif in the Attractin homolog, Distracted, is required for association with Drosophila E3-ligase Mgrn1.
复制标题
DOI：
10.17912/micropub.biology.000416
复制
发表时间：
2021
影响因子：
--
通讯作者：
Godenschwege TA
中科院分区：
文献类型：
--
作者：
Nawaratne V;Kudumala S;Kakad PP;Godenschwege TA
在啮齿类动物中，Attractin（Atrn）跨膜蛋白家族的所有三种旁系同源物都表现出强烈的表型重叠，并与E3连接酶Mahogunin环指1（Mgrn 1）相同的G蛋白偶联受体（GPCR）的调节有关。最近研究表明，哺乳动物中高度保守的细胞内MASRPF基序多表皮生长因子样结构域8蛋白是Mgrn 1结合所需的，以介导体外GPCR Smoothened的遍在化。在这里，我们表明，MASRPF基序的果蝇分心，ATRN和Attractin样1的直系同源物，是必需的协会与果蝇Mgrn1（dMgrn1）在体内。
In rodents, all three paralogs of the Attractin (Atrn) transmembrane protein family exhibit strong phenotypic overlap and are implicated in the regulation of the same G-protein coupled receptors (GPCR) as E3-ligase Mahogunin ring finger 1 (Mgrn1). Recently it was shown that the highly conserved intracellular MASRPF motif in mammal Multiple epidermal growth factor-like domain 8 protein is required for binding of Mgrn1 to mediate ubiquitination of GPCR Smoothened in vitro. Here, we show that the MASRPF motif of Drosophila Distracted, the ortholog of ATRN and Attractin-like 1, is required for association with Drosophila Mgrn1 (dMgrn1) in vivo.