Viable but nonculturable Vibrio cholerae O1 in the aquatic environment of Argentina
Viable but nonculturable Vibrio cholerae O1 in the aquatic environment of Argentina
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DOI：
10.1128/aem.70.12.7481-7486.2004
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发表时间：
2004-12-01
影响因子：
4.4
通讯作者：
Colwell, R
中科院分区：
文献类型：
--
作者：
Binsztein, N;Costagliola, MC;Colwell, R
在阿根廷，如同在拉丁美洲其他国家一样，霍乱已成为一种流行病。霍乱弧菌O 1原产于水生环境，它以可培养和可存活但不可培养（VNC）的形式出现，后者在流行间期出现。这是第一次报告的存在VNC V.霍乱弧菌O 1在河口和海洋沃茨的拉普拉塔和阿根廷大陆架的大西洋，分别。采用免疫荧光和PCR方法，我们能够检测水库的霍乱弧菌O 1携带毒力相关基因ctrA和tcpA。在水、浮游植物和浮游动物样品中鉴定出V cholesterol O 1的VNC形式;浮游动物主要是桡足类Acartia tonsa，Diaptomus sp.，粗吻拟哲水蚤和小拟哲水蚤。我们发现，在有利的条件下，V型霍乱的VNC形式可以恢复到致病的、可传播的状态。我们的结论是，霍乱弧菌O 1是阿根廷沃茨的居民，因为已被证明是在世界其他地理区域的情况。
In Argentina, as in other countries of Latin America, cholera has occurred in an epidemic pattern. Vibrio cholerae O1 is native to the aquatic environment, and it occurs in both culturable and viable but nonculturable (VNC) forms, the latter during interepidemic periods. This is the first report of the presence of VNC V. cholerae O1 in the estuarine and marine waters of the Rio de la Plata and the Argentine shelf of the Atlantic Ocean, respectively. Employing immunofluorescence and PCR methods, we were able to detect reservoirs of V. cholerae O1 carrying the virulence-associated genes ctrA and tcpA. The VNC forms of V cholerae O1 were identified in samples of water, phytoplankton, and zooplankton; the latter organisms were mainly the copepods Acartia tonsa, Diaptomus sp., Paracalanus crassirostris, and Paracalanus parvus. We found that under favorable conditions, the VNC form of V cholerae can revert to the pathogenic, transmissible state. We concluded that V. cholerae O1 is a resident of Argentinean waters, as has been shown to be the case in other geographic regions of the world.