METHODS FOR STUDY OF GLYCOPROTEINS AND PROTEOGLYCANS OF BONE USING BACTERIAL COLLAGENASE - DETERMINATION OF BONE SIALOPROTEIN AND CHONDROITIN SULFATE
METHODS FOR STUDY OF GLYCOPROTEINS AND PROTEOGLYCANS OF BONE USING BACTERIAL COLLAGENASE - DETERMINATION OF BONE SIALOPROTEIN AND CHONDROITIN SULFATE
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DOI：
10.1007/bf02223293
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
HERRING, GM
中科院分区：
文献类型：
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作者：
HERRING, GM
开发了一种使用细菌[溶组织梭菌]胶原酶[EC 3.4.243]溶解骨[牛]基质的方法。可溶性大分子分离的柱程序上的DEAE-纤维素和糖醛酸和唾液酸分析。获得骨的总非胶原部分及其骨唾液蛋白和硫酸软骨素含量的值。将该方法与使用EDTA提取和木瓜蛋白酶[EC 3.4.4.10]消化的类似程序进行比较。
A method was developed for the solubilization of bone [bovine] matrix using bacterial [from Clostridium histolyticum] collagenase [EC 3.4.243]. The soluble macromolecules were separated by a column procedure on DEAE-cellulose and analysed for uronic and sialic acids. Values for the total non-collagenous fraction of bone and its content of bone sialoprotein and chondroitin sulfate were obtained. The method was compared with similar procedures using EDTA extraction and papain [EC 3.4.4.10] digestion.