Changes in Lymphocyte Subsets and Cytokines During European Porcine Reproductive and Respiratory Syndrome: Increased Expression of IL-12 and IL-10 and Proliferation of CD4-CD8high
Changes in Lymphocyte Subsets and Cytokines During European Porcine Reproductive and Respiratory Syndrome: Increased Expression of IL-12 and IL-10 and Proliferation of CD4-CD8high
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DOI：
10.1089/vim.2009.0003
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发表时间：
2009-07-01
期刊：
影响因子：
2.2
通讯作者：
Carrasco, Librado
中科院分区：
文献类型：
--
作者：
Gomez-Laguna, Jaime;Salguero, Francisco J.;Carrasco, Librado
在一些报道中已经研究了外周血单个核细胞（PBMC）的变化，试图确定针对猪繁殖与呼吸综合征病毒（PRRSV）感染的免疫应答。然而，这些变化是如何引起的PRRSV感染后尚未澄清。本研究的目的是分析观察到的变化，在淋巴细胞亚群和免疫调节细胞因子表达的猪急性实验感染后，欧洲的繁殖与呼吸综合征病毒现场分离。猪肌内接种PRRSV田间分离株2982。在不同时间点采集血液、咽后内侧淋巴结和气管支气管淋巴结以及脾脏样本，用于流式细胞术研究，并通过ELISA检测细胞因子表达。从17到24 dpi，PBMC和气管支气管淋巴结细胞中的CD 21（+）细胞计数增加，与血液中PRRSV特异性抗体滴度的增加一致。CD 3（+）T细胞计数增加主要是由于CD 4（-）CD 8（高）和CD 4（+）CD 8（+）T细胞增加。在研究的所有器官中，CD 4（-）CD 8（低）T细胞减少，而CD 4（+）CD 8（-）T细胞仅在脾脏中减少。病毒血症的下降与CD 4（-）CD 8（高）T细胞的增强以及白细胞介素-10（IL-10）和白细胞介素-12 p40（IL-12 p40）的较高表达相关。在感染的急性期没有检测到有效的干扰素-γ（IFN-γ）应答，并且干扰素-α（IFN-α）的表达较晚，并且一旦病毒血症降低就达到其最大表达。这些结果表明IL-10和IL-12作为细胞因子可能在PRRSV免疫应答中起重要作用，CD 4（-）CD 8（高）T细胞也是如此。
The changes in peripheral blood mononuclear cells (PBMCs) have been studied in several reports in an attempt to determine the immune response against porcine reproductive and respiratory syndrome virus (PRRSV) infection. However, how these changes are evoked after PRRSV infection has not yet been clarified. The aim of this study was to analyze the changes seen in lymphocyte subsets and immunomodulatory cytokine expression in pigs after an acute experimental infection with a European PRRSV field isolate. Pigs were inoculated intra-muscularly with PRRSV field isolate 2982. Samples from blood, medial retropharyngeal and tracheobronchial lymph nodes, and spleen were collected at different time points for flow cytometry studies and for cytokine expression by ELISA. CD21(+) cell counts increased in PBMCs and tracheobronchial lymph node cells from 17 to 24 dpi, coinciding with an increase in PRRSV-specific antibody titer in blood. CD3(+) T-cell counts increased mainly due to an enhancement of CD4(-)CD8(high) and CD4(+)CD8(+) T cells. CD4(-)CD8(low) T cells were decreased in all organs studied, whereas CD4(+)CD8(-) T cells decreased only in the spleen. The drop in viremia correlated with an enhancement of CD4(-)CD8(high) T cells, and with a higher expression of interleukin-10 (IL-10) and interleukin-12 p40 (IL-12 p40). No efficient interferon-gamma (IFN-gamma) response was detected during the acute phase of the infection, and the expression of interferon-alpha (IFN-alpha) was late and reached its maximum expression once the viremia decreased. These results point to IL-10 and IL-12 as cytokines that might play a significant role in the PRRSV immune response, as may CD4(-)CD8(high) T cells.