Asymmetric division of clonal muscle stem cells coordinates muscle regeneration in vivo
Asymmetric division of clonal muscle stem cells coordinates muscle regeneration in vivo
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DOI：
10.1126/science.aad9969
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发表时间：
2016-07-08
期刊：
影响因子：
56.9
通讯作者：
Currie, Peter D.
中科院分区：
文献类型：
--
作者：
Gurevich, David B.;Phong Dang Nguyen;Currie, Peter D.
骨骼肌是一个例子，组织部署自我更新的干细胞，卫星细胞，以实现再生。最近的体外研究强调了不对称分裂在更新罕见的“永生”干细胞和产生具有分化能力的成肌细胞克隆群体中的作用。然而，该模型目前缺乏体内验证。我们定义了一个类似于哺乳动物卫星细胞的斑马鱼肌肉干细胞群，并在体内对肌肉从损伤到纤维替代的整个再生过程进行了成像。该分析揭示了卫星细胞与损伤和未损伤纤维之间的复杂相互作用，并提供了通过克隆限制的祖细胞库驱动自我更新和再生的卫星细胞不对称分裂的体内证据。
Skeletal muscle is an example of a tissue that deploys a self-renewing stem cell, the satellite cell, to effect regeneration. Recent in vitro studies have highlighted a role for asymmetric divisions in renewing rare "immortal" stem cells and generating a clonal population of differentiation-competent myoblasts. However, this model currently lacks in vivo validation. We define a zebrafish muscle stem cell population analogous to the mammalian satellite cell and image the entire process of muscle regeneration from injury to fiber replacement in vivo. This analysis reveals complex interactions between satellite cells and both injured and uninjured fibers and provides in vivo evidence for the asymmetric division of satellite cells driving both self-renewal and regeneration via a clonally restricted progenitor pool.